Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms

Detalhes bibliográficos
Autor(a) principal: Cesari,Italo M.
Data de Publicação: 1989
Outros Autores: Aldana,Elis, Gil,Francisco, Condrescu,Madalina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300005
Resumo: A Ca-stimulated ATPase activity (pH 9.5) associated with the tegumental membrane enriched (TME) fraction of Schistosoma mansoni adults was partially inhibited by NAP-taurine or by increasing concentrations of chlorpromazine; endogenous calmodulin was found associated with the TME fraction. A similar activity (pH 8.6) was histochemically visualized whithin the tegument of fixed worms on the cytoplasmic leaflet of both the doubel surface membrane and the basement membrane; this reaction was inhibited by 1 µM chloropromazine and it was also observed on the inner side of double membrane vesicles present in the TME fraction. No ATPase activity could be seen at alkaline pH with added Mg or Na/K ions. Without ATP, the addition of external Ca to the fixed worms induced the appearance of lead precipitates on the tegumental discoid bodies; this reaction was inhibited by molybdate and not by chlorpromazine. The intrategumentary regulation of calcium by the systems described and the possible use of phenothiazines against schistosimes are discussed.
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spelling Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni wormsSchistosoma mansonitegumental membranesdiscoid bodiesCa-ATPaseCalmodulinNAP-taurine inhibitionchlorpromazine inhibitionA Ca-stimulated ATPase activity (pH 9.5) associated with the tegumental membrane enriched (TME) fraction of Schistosoma mansoni adults was partially inhibited by NAP-taurine or by increasing concentrations of chlorpromazine; endogenous calmodulin was found associated with the TME fraction. A similar activity (pH 8.6) was histochemically visualized whithin the tegument of fixed worms on the cytoplasmic leaflet of both the doubel surface membrane and the basement membrane; this reaction was inhibited by 1 µM chloropromazine and it was also observed on the inner side of double membrane vesicles present in the TME fraction. No ATPase activity could be seen at alkaline pH with added Mg or Na/K ions. Without ATP, the addition of external Ca to the fixed worms induced the appearance of lead precipitates on the tegumental discoid bodies; this reaction was inhibited by molybdate and not by chlorpromazine. The intrategumentary regulation of calcium by the systems described and the possible use of phenothiazines against schistosimes are discussed.Instituto Oswaldo Cruz, Ministério da Saúde1989-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300005Memórias do Instituto Oswaldo Cruz v.84 n.3 1989reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761989000300005info:eu-repo/semantics/openAccessCesari,Italo M.Aldana,ElisGil,FranciscoCondrescu,Madalinaeng2020-04-25T17:46:16Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:58.975Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
title Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
spellingShingle Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
Cesari,Italo M.
Schistosoma mansoni
tegumental membranes
discoid bodies
Ca-ATPase
Calmodulin
NAP-taurine inhibition
chlorpromazine inhibition
title_short Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
title_full Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
title_fullStr Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
title_full_unstemmed Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
title_sort Tegumental Ca-stimulated adenosine triphosphatase activity in adult Schistosoma mansoni worms
author Cesari,Italo M.
author_facet Cesari,Italo M.
Aldana,Elis
Gil,Francisco
Condrescu,Madalina
author_role author
author2 Aldana,Elis
Gil,Francisco
Condrescu,Madalina
author2_role author
author
author
dc.contributor.author.fl_str_mv Cesari,Italo M.
Aldana,Elis
Gil,Francisco
Condrescu,Madalina
dc.subject.por.fl_str_mv Schistosoma mansoni
tegumental membranes
discoid bodies
Ca-ATPase
Calmodulin
NAP-taurine inhibition
chlorpromazine inhibition
topic Schistosoma mansoni
tegumental membranes
discoid bodies
Ca-ATPase
Calmodulin
NAP-taurine inhibition
chlorpromazine inhibition
dc.description.none.fl_txt_mv A Ca-stimulated ATPase activity (pH 9.5) associated with the tegumental membrane enriched (TME) fraction of Schistosoma mansoni adults was partially inhibited by NAP-taurine or by increasing concentrations of chlorpromazine; endogenous calmodulin was found associated with the TME fraction. A similar activity (pH 8.6) was histochemically visualized whithin the tegument of fixed worms on the cytoplasmic leaflet of both the doubel surface membrane and the basement membrane; this reaction was inhibited by 1 µM chloropromazine and it was also observed on the inner side of double membrane vesicles present in the TME fraction. No ATPase activity could be seen at alkaline pH with added Mg or Na/K ions. Without ATP, the addition of external Ca to the fixed worms induced the appearance of lead precipitates on the tegumental discoid bodies; this reaction was inhibited by molybdate and not by chlorpromazine. The intrategumentary regulation of calcium by the systems described and the possible use of phenothiazines against schistosimes are discussed.
description A Ca-stimulated ATPase activity (pH 9.5) associated with the tegumental membrane enriched (TME) fraction of Schistosoma mansoni adults was partially inhibited by NAP-taurine or by increasing concentrations of chlorpromazine; endogenous calmodulin was found associated with the TME fraction. A similar activity (pH 8.6) was histochemically visualized whithin the tegument of fixed worms on the cytoplasmic leaflet of both the doubel surface membrane and the basement membrane; this reaction was inhibited by 1 µM chloropromazine and it was also observed on the inner side of double membrane vesicles present in the TME fraction. No ATPase activity could be seen at alkaline pH with added Mg or Na/K ions. Without ATP, the addition of external Ca to the fixed worms induced the appearance of lead precipitates on the tegumental discoid bodies; this reaction was inhibited by molybdate and not by chlorpromazine. The intrategumentary regulation of calcium by the systems described and the possible use of phenothiazines against schistosimes are discussed.
publishDate 1989
dc.date.none.fl_str_mv 1989-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761989000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.84 n.3 1989
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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