Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018 |
Resumo: | The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods. |
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Memórias do Instituto Oswaldo Cruz |
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Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centreLeishmaniapolymerase chain reactiondiagnosisThe present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.Instituto Oswaldo Cruz, Ministério da Saúde2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018Memórias do Instituto Oswaldo Cruz v.105 n.1 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000100018info:eu-repo/semantics/openAccessFagundes,AlineSchubach,ArmandoPaula,Cíntia Cristiane deBogio,AlessandraAntonio,Liliane de FátimaSchiavoni,Patrícia BotelhoMonteiro,Vivian de SouzaMadeira,Maria de FátimaQuintella,Leonardo PereiraValete-Rosalino,Claudia MariaVasconcellos,Erica de Camargo Ferreira eAzeredo-Coutinho,Rilza Beatriz Gayoso dePacheco,Rachel SMarzochi,Mauro CAMarzochi,Keyla BFeng2020-04-25T17:50:45Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:47.977Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
title |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
spellingShingle |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre Fagundes,Aline Leishmania polymerase chain reaction diagnosis |
title_short |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
title_full |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
title_fullStr |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
title_full_unstemmed |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
title_sort |
Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre |
author |
Fagundes,Aline |
author_facet |
Fagundes,Aline Schubach,Armando Paula,Cíntia Cristiane de Bogio,Alessandra Antonio,Liliane de Fátima Schiavoni,Patrícia Botelho Monteiro,Vivian de Souza Madeira,Maria de Fátima Quintella,Leonardo Pereira Valete-Rosalino,Claudia Maria Vasconcellos,Erica de Camargo Ferreira e Azeredo-Coutinho,Rilza Beatriz Gayoso de Pacheco,Rachel S Marzochi,Mauro CA Marzochi,Keyla BF |
author_role |
author |
author2 |
Schubach,Armando Paula,Cíntia Cristiane de Bogio,Alessandra Antonio,Liliane de Fátima Schiavoni,Patrícia Botelho Monteiro,Vivian de Souza Madeira,Maria de Fátima Quintella,Leonardo Pereira Valete-Rosalino,Claudia Maria Vasconcellos,Erica de Camargo Ferreira e Azeredo-Coutinho,Rilza Beatriz Gayoso de Pacheco,Rachel S Marzochi,Mauro CA Marzochi,Keyla BF |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fagundes,Aline Schubach,Armando Paula,Cíntia Cristiane de Bogio,Alessandra Antonio,Liliane de Fátima Schiavoni,Patrícia Botelho Monteiro,Vivian de Souza Madeira,Maria de Fátima Quintella,Leonardo Pereira Valete-Rosalino,Claudia Maria Vasconcellos,Erica de Camargo Ferreira e Azeredo-Coutinho,Rilza Beatriz Gayoso de Pacheco,Rachel S Marzochi,Mauro CA Marzochi,Keyla BF |
dc.subject.por.fl_str_mv |
Leishmania polymerase chain reaction diagnosis |
topic |
Leishmania polymerase chain reaction diagnosis |
dc.description.none.fl_txt_mv |
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods. |
description |
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762010000100018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.105 n.1 2010 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937706902224896 |