Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre

Detalhes bibliográficos
Autor(a) principal: Fagundes,Aline
Data de Publicação: 2010
Outros Autores: Schubach,Armando, Paula,Cíntia Cristiane de, Bogio,Alessandra, Antonio,Liliane de Fátima, Schiavoni,Patrícia Botelho, Monteiro,Vivian de Souza, Madeira,Maria de Fátima, Quintella,Leonardo Pereira, Valete-Rosalino,Claudia Maria, Vasconcellos,Erica de Camargo Ferreira e, Azeredo-Coutinho,Rilza Beatriz Gayoso de, Pacheco,Rachel S, Marzochi,Mauro CA, Marzochi,Keyla BF
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018
Resumo: The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
id FIOCRUZ-4_1f8aee184b4414a8256f06107aa8270f
oai_identifier_str oai:scielo:S0074-02762010000100018
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centreLeishmaniapolymerase chain reactiondiagnosisThe present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.Instituto Oswaldo Cruz, Ministério da Saúde2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018Memórias do Instituto Oswaldo Cruz v.105 n.1 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000100018info:eu-repo/semantics/openAccessFagundes,AlineSchubach,ArmandoPaula,Cíntia Cristiane deBogio,AlessandraAntonio,Liliane de FátimaSchiavoni,Patrícia BotelhoMonteiro,Vivian de SouzaMadeira,Maria de FátimaQuintella,Leonardo PereiraValete-Rosalino,Claudia MariaVasconcellos,Erica de Camargo Ferreira eAzeredo-Coutinho,Rilza Beatriz Gayoso dePacheco,Rachel SMarzochi,Mauro CAMarzochi,Keyla BFeng2020-04-25T17:50:45Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:47.977Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
title Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
spellingShingle Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
Fagundes,Aline
Leishmania
polymerase chain reaction
diagnosis
title_short Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
title_full Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
title_fullStr Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
title_full_unstemmed Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
title_sort Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
author Fagundes,Aline
author_facet Fagundes,Aline
Schubach,Armando
Paula,Cíntia Cristiane de
Bogio,Alessandra
Antonio,Liliane de Fátima
Schiavoni,Patrícia Botelho
Monteiro,Vivian de Souza
Madeira,Maria de Fátima
Quintella,Leonardo Pereira
Valete-Rosalino,Claudia Maria
Vasconcellos,Erica de Camargo Ferreira e
Azeredo-Coutinho,Rilza Beatriz Gayoso de
Pacheco,Rachel S
Marzochi,Mauro CA
Marzochi,Keyla BF
author_role author
author2 Schubach,Armando
Paula,Cíntia Cristiane de
Bogio,Alessandra
Antonio,Liliane de Fátima
Schiavoni,Patrícia Botelho
Monteiro,Vivian de Souza
Madeira,Maria de Fátima
Quintella,Leonardo Pereira
Valete-Rosalino,Claudia Maria
Vasconcellos,Erica de Camargo Ferreira e
Azeredo-Coutinho,Rilza Beatriz Gayoso de
Pacheco,Rachel S
Marzochi,Mauro CA
Marzochi,Keyla BF
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fagundes,Aline
Schubach,Armando
Paula,Cíntia Cristiane de
Bogio,Alessandra
Antonio,Liliane de Fátima
Schiavoni,Patrícia Botelho
Monteiro,Vivian de Souza
Madeira,Maria de Fátima
Quintella,Leonardo Pereira
Valete-Rosalino,Claudia Maria
Vasconcellos,Erica de Camargo Ferreira e
Azeredo-Coutinho,Rilza Beatriz Gayoso de
Pacheco,Rachel S
Marzochi,Mauro CA
Marzochi,Keyla BF
dc.subject.por.fl_str_mv Leishmania
polymerase chain reaction
diagnosis
topic Leishmania
polymerase chain reaction
diagnosis
dc.description.none.fl_txt_mv The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
description The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000100018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.1 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937706902224896