Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum

Detalhes bibliográficos
Autor(a) principal: Prestes,Elisa Beatriz
Data de Publicação: 2012
Outros Autores: Bayer-Santos,Ethel, Hermes Stoco,Patrícia, Sincero,Thaís Cristine Marques, Wagner,Glauber, Umaki,Adriana, Fragoso,Stenio Perdigão, Bordignon,Juliano, Steindel,Mário, Grisard,Edmundo Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600002
Resumo: Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.
id FIOCRUZ-4_27bcb7f72ec91358eca323a92089a95b
oai_identifier_str oai:scielo:S0074-02762012000600002
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellumTrypanosoma rangeliprotein tyrosine phosphataseflagellumProtein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.Instituto Oswaldo Cruz, Ministério da Saúde2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600002Memórias do Instituto Oswaldo Cruz v.107 n.6 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000600002info:eu-repo/semantics/openAccessPrestes,Elisa BeatrizBayer-Santos,EthelHermes Stoco,PatríciaSincero,Thaís Cristine MarquesWagner,GlauberUmaki,AdrianaFragoso,Stenio PerdigãoBordignon,JulianoSteindel,MárioGrisard,Edmundo Carloseng2020-04-25T17:51:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:28.608Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
title Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
spellingShingle Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
Prestes,Elisa Beatriz
Trypanosoma rangeli
protein tyrosine phosphatase
flagellum
title_short Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
title_full Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
title_fullStr Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
title_full_unstemmed Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
title_sort Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum
author Prestes,Elisa Beatriz
author_facet Prestes,Elisa Beatriz
Bayer-Santos,Ethel
Hermes Stoco,Patrícia
Sincero,Thaís Cristine Marques
Wagner,Glauber
Umaki,Adriana
Fragoso,Stenio Perdigão
Bordignon,Juliano
Steindel,Mário
Grisard,Edmundo Carlos
author_role author
author2 Bayer-Santos,Ethel
Hermes Stoco,Patrícia
Sincero,Thaís Cristine Marques
Wagner,Glauber
Umaki,Adriana
Fragoso,Stenio Perdigão
Bordignon,Juliano
Steindel,Mário
Grisard,Edmundo Carlos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Prestes,Elisa Beatriz
Bayer-Santos,Ethel
Hermes Stoco,Patrícia
Sincero,Thaís Cristine Marques
Wagner,Glauber
Umaki,Adriana
Fragoso,Stenio Perdigão
Bordignon,Juliano
Steindel,Mário
Grisard,Edmundo Carlos
dc.subject.por.fl_str_mv Trypanosoma rangeli
protein tyrosine phosphatase
flagellum
topic Trypanosoma rangeli
protein tyrosine phosphatase
flagellum
dc.description.none.fl_txt_mv Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.
description Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600002
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000600002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.6 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937712111550464

Registros relacionados