A comparative study of preservation and storage of Haemophilus influenzae

Detalhes bibliográficos
Autor(a) principal: Saab,Olga C Aulet de
Data de Publicação: 2001
Outros Autores: Castillo,Marta C de, Ruiz Holgado,Aida P de, Nader,Olga M de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022
Resumo: The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.
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spelling A comparative study of preservation and storage of Haemophilus influenzaeHaemophilus influenzaestorageculture mediaThe aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.Instituto Oswaldo Cruz, Ministério da Saúde2001-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022Memórias do Instituto Oswaldo Cruz v.96 n.4 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000400022info:eu-repo/semantics/openAccessSaab,Olga C Aulet deCastillo,Marta C deRuiz Holgado,Aida P deNader,Olga M deeng2020-04-25T17:48:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:21.869Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A comparative study of preservation and storage of Haemophilus influenzae
title A comparative study of preservation and storage of Haemophilus influenzae
spellingShingle A comparative study of preservation and storage of Haemophilus influenzae
Saab,Olga C Aulet de
Haemophilus influenzae
storage
culture media
title_short A comparative study of preservation and storage of Haemophilus influenzae
title_full A comparative study of preservation and storage of Haemophilus influenzae
title_fullStr A comparative study of preservation and storage of Haemophilus influenzae
title_full_unstemmed A comparative study of preservation and storage of Haemophilus influenzae
title_sort A comparative study of preservation and storage of Haemophilus influenzae
author Saab,Olga C Aulet de
author_facet Saab,Olga C Aulet de
Castillo,Marta C de
Ruiz Holgado,Aida P de
Nader,Olga M de
author_role author
author2 Castillo,Marta C de
Ruiz Holgado,Aida P de
Nader,Olga M de
author2_role author
author
author
dc.contributor.author.fl_str_mv Saab,Olga C Aulet de
Castillo,Marta C de
Ruiz Holgado,Aida P de
Nader,Olga M de
dc.subject.por.fl_str_mv Haemophilus influenzae
storage
culture media
topic Haemophilus influenzae
storage
culture media
dc.description.none.fl_txt_mv The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.
description The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.
publishDate 2001
dc.date.none.fl_str_mv 2001-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000400022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 n.4 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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