Symbiont modulates expression of specific gene categories in Angomonas deanei
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100686 |
Resumo: | Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei. |
id |
FIOCRUZ-4_3224dbaa2bead2f31b77fce132dbceb0 |
---|---|
oai_identifier_str |
oai:scielo:S0074-02762016001100686 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Symbiont modulates expression of specific gene categories in Angomonas deaneitrypanosomatid RNA-seqgene expressionaposymbiotic strainendosymbiosisTrypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei.Instituto Oswaldo Cruz, Ministério da Saúde2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100686Memórias do Instituto Oswaldo Cruz v.111 n.11 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760160228info:eu-repo/semantics/openAccessPenha,Luciana LoureiroHoffmann,LuísaSouza,Silvanna Sant’Anna deMartins,Allan Cézar de AzevedoBottaro,ThayaneProsdocimi,FranciscoFaffe,Débora SouzaMotta,Maria Cristina MachadoÜrményi,Turán PéterSilva,Rosaneeng2020-04-25T17:52:30Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:27.321Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
title |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
spellingShingle |
Symbiont modulates expression of specific gene categories in Angomonas deanei Penha,Luciana Loureiro trypanosomatid RNA-seq gene expression aposymbiotic strain endosymbiosis |
title_short |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
title_full |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
title_fullStr |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
title_full_unstemmed |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
title_sort |
Symbiont modulates expression of specific gene categories in Angomonas deanei |
author |
Penha,Luciana Loureiro |
author_facet |
Penha,Luciana Loureiro Hoffmann,Luísa Souza,Silvanna Sant’Anna de Martins,Allan Cézar de Azevedo Bottaro,Thayane Prosdocimi,Francisco Faffe,Débora Souza Motta,Maria Cristina Machado Ürményi,Turán Péter Silva,Rosane |
author_role |
author |
author2 |
Hoffmann,Luísa Souza,Silvanna Sant’Anna de Martins,Allan Cézar de Azevedo Bottaro,Thayane Prosdocimi,Francisco Faffe,Débora Souza Motta,Maria Cristina Machado Ürményi,Turán Péter Silva,Rosane |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Penha,Luciana Loureiro Hoffmann,Luísa Souza,Silvanna Sant’Anna de Martins,Allan Cézar de Azevedo Bottaro,Thayane Prosdocimi,Francisco Faffe,Débora Souza Motta,Maria Cristina Machado Ürményi,Turán Péter Silva,Rosane |
dc.subject.por.fl_str_mv |
trypanosomatid RNA-seq gene expression aposymbiotic strain endosymbiosis |
topic |
trypanosomatid RNA-seq gene expression aposymbiotic strain endosymbiosis |
dc.description.none.fl_txt_mv |
Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei. |
description |
Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100686 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100686 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760160228 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.111 n.11 2016 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937722557464576 |