The use of ferromagnetic dacron as solid-phase in enzyme immunoassays

Detalhes bibliográficos
Autor(a) principal: Leão,Ana Maria dos A. Carneiro
Data de Publicação: 1994
Outros Autores: Carvalho Junior,Luiz B., Malagueño,Elizabeth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200012
Resumo: Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
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spelling The use of ferromagnetic dacron as solid-phase in enzyme immunoassaysmagnetic ELISAferromagnetic dacronhuman serum albuminFerromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.Instituto Oswaldo Cruz, Ministério da Saúde1994-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200012Memórias do Instituto Oswaldo Cruz v.89 n.2 1994reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761994000200012info:eu-repo/semantics/openAccessLeão,Ana Maria dos A. CarneiroCarvalho Junior,Luiz B.Malagueño,Elizabetheng2020-04-25T17:47:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:05:56.714Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
title The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
spellingShingle The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
Leão,Ana Maria dos A. Carneiro
magnetic ELISA
ferromagnetic dacron
human serum albumin
title_short The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
title_full The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
title_fullStr The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
title_full_unstemmed The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
title_sort The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
author Leão,Ana Maria dos A. Carneiro
author_facet Leão,Ana Maria dos A. Carneiro
Carvalho Junior,Luiz B.
Malagueño,Elizabeth
author_role author
author2 Carvalho Junior,Luiz B.
Malagueño,Elizabeth
author2_role author
author
dc.contributor.author.fl_str_mv Leão,Ana Maria dos A. Carneiro
Carvalho Junior,Luiz B.
Malagueño,Elizabeth
dc.subject.por.fl_str_mv magnetic ELISA
ferromagnetic dacron
human serum albumin
topic magnetic ELISA
ferromagnetic dacron
human serum albumin
dc.description.none.fl_txt_mv Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
description Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
publishDate 1994
dc.date.none.fl_str_mv 1994-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200012
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761994000200012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.89 n.2 1994
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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