Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004 |
Resumo: | Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. |
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Memórias do Instituto Oswaldo Cruz |
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Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in ThailandAnopheles campestris-likemetaphase karyotypecrossing experimentITS2COICOIISeventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.Instituto Oswaldo Cruz, Ministério da Saúde2009-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004Memórias do Instituto Oswaldo Cruz v.104 n.4 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000400004info:eu-repo/semantics/openAccessThongsahuan,SorawatBaimai,VisutOtsuka,YasushiSaeung,AtipornTuetun,BenjawanJariyapan,NarissaraSuwannamit,SittipornSomboon,PradyaJitpakdi,AtchariyaTakaoka,HiroyukiChoochote,Wejeng2020-04-25T17:50:29Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:13.911Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
spellingShingle |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand Thongsahuan,Sorawat Anopheles campestris-like metaphase karyotype crossing experiment ITS2 COI COII |
title_short |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_full |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_fullStr |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_full_unstemmed |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
title_sort |
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand |
author |
Thongsahuan,Sorawat |
author_facet |
Thongsahuan,Sorawat Baimai,Visut Otsuka,Yasushi Saeung,Atiporn Tuetun,Benjawan Jariyapan,Narissara Suwannamit,Sittiporn Somboon,Pradya Jitpakdi,Atchariya Takaoka,Hiroyuki Choochote,Wej |
author_role |
author |
author2 |
Baimai,Visut Otsuka,Yasushi Saeung,Atiporn Tuetun,Benjawan Jariyapan,Narissara Suwannamit,Sittiporn Somboon,Pradya Jitpakdi,Atchariya Takaoka,Hiroyuki Choochote,Wej |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Thongsahuan,Sorawat Baimai,Visut Otsuka,Yasushi Saeung,Atiporn Tuetun,Benjawan Jariyapan,Narissara Suwannamit,Sittiporn Somboon,Pradya Jitpakdi,Atchariya Takaoka,Hiroyuki Choochote,Wej |
dc.subject.por.fl_str_mv |
Anopheles campestris-like metaphase karyotype crossing experiment ITS2 COI COII |
topic |
Anopheles campestris-like metaphase karyotype crossing experiment ITS2 COI COII |
dc.description.none.fl_txt_mv |
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. |
description |
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762009000400004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.104 n.4 2009 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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_version_ |
1669937705471967232 |