Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Barbosa,Joana
Data de Publicação: 2014
Outros Autores: Rodrigues,Acácio Gonçalves, Pérez,Maria José, Pina-Vaz,Cidália
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801078
Resumo: Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.
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spelling Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometryGiardia duodenalisflow cytometrysusceptibilityGiardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability. Instituto Oswaldo Cruz, Ministério da Saúde2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801078Memórias do Instituto Oswaldo Cruz v.109 n.8 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140267info:eu-repo/semantics/openAccessBarbosa,JoanaRodrigues,Acácio GonçalvesPérez,Maria JoséPina-Vaz,Cidáliaeng2020-04-25T17:51:53Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:09.951Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
title Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
spellingShingle Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
Barbosa,Joana
Giardia duodenalis
flow cytometry
susceptibility
title_short Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
title_full Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
title_fullStr Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
title_full_unstemmed Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
title_sort Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
author Barbosa,Joana
author_facet Barbosa,Joana
Rodrigues,Acácio Gonçalves
Pérez,Maria José
Pina-Vaz,Cidália
author_role author
author2 Rodrigues,Acácio Gonçalves
Pérez,Maria José
Pina-Vaz,Cidália
author2_role author
author
author
dc.contributor.author.fl_str_mv Barbosa,Joana
Rodrigues,Acácio Gonçalves
Pérez,Maria José
Pina-Vaz,Cidália
dc.subject.por.fl_str_mv Giardia duodenalis
flow cytometry
susceptibility
topic Giardia duodenalis
flow cytometry
susceptibility
dc.description.none.fl_txt_mv Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.
description Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801078
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801078
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276140267
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.109 n.8 2014
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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