Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells

Detalhes bibliográficos
Autor(a) principal: Rocha,Fernanda F
Data de Publicação: 2010
Outros Autores: Araujo,Ricardo N, Silva,Luciana M, Gontijo,Nelder F, Pereira,Marcos H
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004
Resumo: In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
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spelling Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cellsRhodnius prolixussalivary glandprimary cultureIn the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.Instituto Oswaldo Cruz, Ministério da Saúde2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004Memórias do Instituto Oswaldo Cruz v.105 n.2 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000200004info:eu-repo/semantics/openAccessRocha,Fernanda FAraujo,Ricardo NSilva,Luciana MGontijo,Nelder FPereira,Marcos Heng2020-04-25T17:50:45Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:49.162Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
title Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
spellingShingle Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
Rocha,Fernanda F
Rhodnius prolixus
salivary gland
primary culture
title_short Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
title_full Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
title_fullStr Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
title_full_unstemmed Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
title_sort Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
author Rocha,Fernanda F
author_facet Rocha,Fernanda F
Araujo,Ricardo N
Silva,Luciana M
Gontijo,Nelder F
Pereira,Marcos H
author_role author
author2 Araujo,Ricardo N
Silva,Luciana M
Gontijo,Nelder F
Pereira,Marcos H
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rocha,Fernanda F
Araujo,Ricardo N
Silva,Luciana M
Gontijo,Nelder F
Pereira,Marcos H
dc.subject.por.fl_str_mv Rhodnius prolixus
salivary gland
primary culture
topic Rhodnius prolixus
salivary gland
primary culture
dc.description.none.fl_txt_mv In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
description In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.2 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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