Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011 |
Resumo: | Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs. |
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Memórias do Instituto Oswaldo Cruz |
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Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burdenswine cysticercosislarval antigensantibodiesenzyme-linked immunosorbent assayimmunoblotSeven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.Instituto Oswaldo Cruz, Ministério da Saúde2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011Memórias do Instituto Oswaldo Cruz v.102 n.6 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000085info:eu-repo/semantics/openAccessGomes,Andréia BartachiniSoares,Killarney AtaídeBueno,Ednéia CasagrandeEspindola,Noeli MariaIha,Alberto HiroshiMaia,Antônio Augusto MendesPeralta,Regina Helena SaramargoVaz,Adelaide Joséeng2020-04-25T17:50:11Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:18.012Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
title |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
spellingShingle |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden Gomes,Andréia Bartachini swine cysticercosis larval antigens antibodies enzyme-linked immunosorbent assay immunoblot |
title_short |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
title_full |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
title_fullStr |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
title_full_unstemmed |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
title_sort |
Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
author |
Gomes,Andréia Bartachini |
author_facet |
Gomes,Andréia Bartachini Soares,Killarney Ataíde Bueno,Ednéia Casagrande Espindola,Noeli Maria Iha,Alberto Hiroshi Maia,Antônio Augusto Mendes Peralta,Regina Helena Saramargo Vaz,Adelaide José |
author_role |
author |
author2 |
Soares,Killarney Ataíde Bueno,Ednéia Casagrande Espindola,Noeli Maria Iha,Alberto Hiroshi Maia,Antônio Augusto Mendes Peralta,Regina Helena Saramargo Vaz,Adelaide José |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes,Andréia Bartachini Soares,Killarney Ataíde Bueno,Ednéia Casagrande Espindola,Noeli Maria Iha,Alberto Hiroshi Maia,Antônio Augusto Mendes Peralta,Regina Helena Saramargo Vaz,Adelaide José |
dc.subject.por.fl_str_mv |
swine cysticercosis larval antigens antibodies enzyme-linked immunosorbent assay immunoblot |
topic |
swine cysticercosis larval antigens antibodies enzyme-linked immunosorbent assay immunoblot |
dc.description.none.fl_txt_mv |
Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs. |
description |
Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762007005000085 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.102 n.6 2007 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937701806145536 |