Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

Detalhes bibliográficos
Autor(a) principal: Gomes,Andréia Bartachini
Data de Publicação: 2007
Outros Autores: Soares,Killarney Ataíde, Bueno,Ednéia Casagrande, Espindola,Noeli Maria, Iha,Alberto Hiroshi, Maia,Antônio Augusto Mendes, Peralta,Regina Helena Saramargo, Vaz,Adelaide José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011
Resumo: Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.
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spelling Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burdenswine cysticercosislarval antigensantibodiesenzyme-linked immunosorbent assayimmunoblotSeven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.Instituto Oswaldo Cruz, Ministério da Saúde2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011Memórias do Instituto Oswaldo Cruz v.102 n.6 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000085info:eu-repo/semantics/openAccessGomes,Andréia BartachiniSoares,Killarney AtaídeBueno,Ednéia CasagrandeEspindola,Noeli MariaIha,Alberto HiroshiMaia,Antônio Augusto MendesPeralta,Regina Helena SaramargoVaz,Adelaide Joséeng2020-04-25T17:50:11Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:18.012Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
title Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
spellingShingle Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
Gomes,Andréia Bartachini
swine cysticercosis
larval antigens
antibodies
enzyme-linked immunosorbent assay
immunoblot
title_short Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
title_full Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
title_fullStr Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
title_full_unstemmed Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
title_sort Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
author Gomes,Andréia Bartachini
author_facet Gomes,Andréia Bartachini
Soares,Killarney Ataíde
Bueno,Ednéia Casagrande
Espindola,Noeli Maria
Iha,Alberto Hiroshi
Maia,Antônio Augusto Mendes
Peralta,Regina Helena Saramargo
Vaz,Adelaide José
author_role author
author2 Soares,Killarney Ataíde
Bueno,Ednéia Casagrande
Espindola,Noeli Maria
Iha,Alberto Hiroshi
Maia,Antônio Augusto Mendes
Peralta,Regina Helena Saramargo
Vaz,Adelaide José
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes,Andréia Bartachini
Soares,Killarney Ataíde
Bueno,Ednéia Casagrande
Espindola,Noeli Maria
Iha,Alberto Hiroshi
Maia,Antônio Augusto Mendes
Peralta,Regina Helena Saramargo
Vaz,Adelaide José
dc.subject.por.fl_str_mv swine cysticercosis
larval antigens
antibodies
enzyme-linked immunosorbent assay
immunoblot
topic swine cysticercosis
larval antigens
antibodies
enzyme-linked immunosorbent assay
immunoblot
dc.description.none.fl_txt_mv Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.
description Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762007005000085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.102 n.6 2007
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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