Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development

Detalhes bibliográficos
Autor(a) principal: Weerasekera,Manjula M
Data de Publicação: 2016
Outros Autores: Wijesinghe,Gayan K, Jayarathna,Thilini A, Gunasekara,Chinthika P, Fernando,Neluka, Kottegoda,Nilwala, Samaranayake,Lakshman P
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100697
Resumo: As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.
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spelling Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm developmentCandida biofilmsculture mediaSDBYNBRPMI 1640As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.Instituto Oswaldo Cruz, Ministério da Saúde2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100697Memórias do Instituto Oswaldo Cruz v.111 n.11 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760160294info:eu-repo/semantics/openAccessWeerasekera,Manjula MWijesinghe,Gayan KJayarathna,Thilini AGunasekara,Chinthika PFernando,NelukaKottegoda,NilwalaSamaranayake,Lakshman Peng2020-04-25T17:52:30Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:27.51Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
title Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
spellingShingle Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
Weerasekera,Manjula M
Candida biofilms
culture media
SDB
YNB
RPMI 1640
title_short Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
title_full Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
title_fullStr Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
title_full_unstemmed Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
title_sort Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
author Weerasekera,Manjula M
author_facet Weerasekera,Manjula M
Wijesinghe,Gayan K
Jayarathna,Thilini A
Gunasekara,Chinthika P
Fernando,Neluka
Kottegoda,Nilwala
Samaranayake,Lakshman P
author_role author
author2 Wijesinghe,Gayan K
Jayarathna,Thilini A
Gunasekara,Chinthika P
Fernando,Neluka
Kottegoda,Nilwala
Samaranayake,Lakshman P
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Weerasekera,Manjula M
Wijesinghe,Gayan K
Jayarathna,Thilini A
Gunasekara,Chinthika P
Fernando,Neluka
Kottegoda,Nilwala
Samaranayake,Lakshman P
dc.subject.por.fl_str_mv Candida biofilms
culture media
SDB
YNB
RPMI 1640
topic Candida biofilms
culture media
SDB
YNB
RPMI 1640
dc.description.none.fl_txt_mv As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.
description As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100697
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001100697
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760160294
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.111 n.11 2016
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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