DNA polymorphism of schistosomes and their snail hosts

Detalhes bibliográficos
Autor(a) principal: Simpson,Andrew J. G.
Data de Publicação: 1995
Outros Autores: Dias Neto,Emmanuel, Vidigal,Teofania H. D. A., Pena,Heloísa B., Carvalho,Omar S., Pena,Sergio D. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200014
Resumo: Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.
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spelling DNA polymorphism of schistosomes and their snail hostsschistosomesBiomphalaria glabrataDNApolymorphismsPCRAnalysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.Instituto Oswaldo Cruz, Ministério da Saúde1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200014Memórias do Instituto Oswaldo Cruz v.90 n.2 1995reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761995000200014info:eu-repo/semantics/openAccessSimpson,Andrew J. G.Dias Neto,EmmanuelVidigal,Teofania H. D. A.Pena,Heloísa B.Carvalho,Omar S.Pena,Sergio D. J.eng2020-04-25T17:47:23Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:27.12Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv DNA polymorphism of schistosomes and their snail hosts
title DNA polymorphism of schistosomes and their snail hosts
spellingShingle DNA polymorphism of schistosomes and their snail hosts
Simpson,Andrew J. G.
schistosomes
Biomphalaria glabrata
DNA
polymorphisms
PCR
title_short DNA polymorphism of schistosomes and their snail hosts
title_full DNA polymorphism of schistosomes and their snail hosts
title_fullStr DNA polymorphism of schistosomes and their snail hosts
title_full_unstemmed DNA polymorphism of schistosomes and their snail hosts
title_sort DNA polymorphism of schistosomes and their snail hosts
author Simpson,Andrew J. G.
author_facet Simpson,Andrew J. G.
Dias Neto,Emmanuel
Vidigal,Teofania H. D. A.
Pena,Heloísa B.
Carvalho,Omar S.
Pena,Sergio D. J.
author_role author
author2 Dias Neto,Emmanuel
Vidigal,Teofania H. D. A.
Pena,Heloísa B.
Carvalho,Omar S.
Pena,Sergio D. J.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Simpson,Andrew J. G.
Dias Neto,Emmanuel
Vidigal,Teofania H. D. A.
Pena,Heloísa B.
Carvalho,Omar S.
Pena,Sergio D. J.
dc.subject.por.fl_str_mv schistosomes
Biomphalaria glabrata
DNA
polymorphisms
PCR
topic schistosomes
Biomphalaria glabrata
DNA
polymorphisms
PCR
dc.description.none.fl_txt_mv Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.
description Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.
publishDate 1995
dc.date.none.fl_str_mv 1995-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200014
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761995000200014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.90 n.2 1995
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937664058458112

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