In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Memórias do Instituto Oswaldo Cruz |
| Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300147 |
Resumo: | The polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis. |
| id |
FIOCRUZ-4_5871d05bddca6f53a76ed4c6f84a0ddd |
|---|---|
| oai_identifier_str |
oai:scielo:S0074-02762016000300147 |
| network_acronym_str |
FIOCRUZ-4 |
| network_name_str |
Memórias do Instituto Oswaldo Cruz |
| spelling |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowiiantileishmanial activityplant extractsnatural productsexperimental leishmaniasisThe polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis.Instituto Oswaldo Cruz, Ministério da Saúde2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300147Memórias do Instituto Oswaldo Cruz v.111 n.3 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150307info:eu-repo/semantics/openAccessQueiroz,Dayane Priscilla de SouzaCarollo,Carlos AlexandreKadri,Mônica Cristina ToffoliRizk,Yasmin SilvaAraujo,Vanessa Carneiro Pereira deMonteiro,Paulo Eduardo de OliveiraRodrigues,Patrik OeningOshiro,Elisa TeruyaMatos,Maria de Fátima CepaArruda,Carla Cardozo Pinto deeng2020-04-25T17:52:27Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:21.12Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
| dc.title.none.fl_str_mv |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| title |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| spellingShingle |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii Queiroz,Dayane Priscilla de Souza antileishmanial activity plant extracts natural products experimental leishmaniasis |
| title_short |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| title_full |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| title_fullStr |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| title_full_unstemmed |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| title_sort |
In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii |
| author |
Queiroz,Dayane Priscilla de Souza |
| author_facet |
Queiroz,Dayane Priscilla de Souza Carollo,Carlos Alexandre Kadri,Mônica Cristina Toffoli Rizk,Yasmin Silva Araujo,Vanessa Carneiro Pereira de Monteiro,Paulo Eduardo de Oliveira Rodrigues,Patrik Oening Oshiro,Elisa Teruya Matos,Maria de Fátima Cepa Arruda,Carla Cardozo Pinto de |
| author_role |
author |
| author2 |
Carollo,Carlos Alexandre Kadri,Mônica Cristina Toffoli Rizk,Yasmin Silva Araujo,Vanessa Carneiro Pereira de Monteiro,Paulo Eduardo de Oliveira Rodrigues,Patrik Oening Oshiro,Elisa Teruya Matos,Maria de Fátima Cepa Arruda,Carla Cardozo Pinto de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Queiroz,Dayane Priscilla de Souza Carollo,Carlos Alexandre Kadri,Mônica Cristina Toffoli Rizk,Yasmin Silva Araujo,Vanessa Carneiro Pereira de Monteiro,Paulo Eduardo de Oliveira Rodrigues,Patrik Oening Oshiro,Elisa Teruya Matos,Maria de Fátima Cepa Arruda,Carla Cardozo Pinto de |
| dc.subject.por.fl_str_mv |
antileishmanial activity plant extracts natural products experimental leishmaniasis |
| topic |
antileishmanial activity plant extracts natural products experimental leishmaniasis |
| dc.description.none.fl_txt_mv |
The polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis. |
| description |
The polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-03-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300147 |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300147 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/0074-02760150307 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.111 n.3 2016 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
| reponame_str |
Memórias do Instituto Oswaldo Cruz |
| collection |
Memórias do Instituto Oswaldo Cruz |
| instname_str |
Fundação Oswaldo Cruz |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
| repository.mail.fl_str_mv |
|
| _version_ |
1669937721311756288 |