Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis

Detalhes bibliográficos
Autor(a) principal: Timm,Solange L.
Data de Publicação: 1980
Outros Autores: Leon,Leonor L., Pereira,Neize M., Souza,Wanderley de, Cruz,M. Queiroz, Bräscher,Helena M., Lima,A. Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761980000100014
Resumo: A cell fractionation procedure previously developed for Trypanosoma cruzi was applied to isolated the plasma membrane of promastigotes of Leishania mexicana amazonensis. The cell, swollen in an hypotonic mediun, were disrupted in the presence of a nonionic detergent and the membrane fraction isolated by differencial centrifugation. Electron microscopy showed that the fraction consisted of pieces of the plasma membrane associated with subpellicular microtubules. It was also shown that this fraction is able to induce cell-mediated immune response in mice.
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spelling Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensisA cell fractionation procedure previously developed for Trypanosoma cruzi was applied to isolated the plasma membrane of promastigotes of Leishania mexicana amazonensis. The cell, swollen in an hypotonic mediun, were disrupted in the presence of a nonionic detergent and the membrane fraction isolated by differencial centrifugation. Electron microscopy showed that the fraction consisted of pieces of the plasma membrane associated with subpellicular microtubules. It was also shown that this fraction is able to induce cell-mediated immune response in mice.Instituto Oswaldo Cruz, Ministério da Saúde1980-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761980000100014Memórias do Instituto Oswaldo Cruz v.75 n.1-2 1980reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761980000100014info:eu-repo/semantics/openAccessTimm,Solange L.Leon,Leonor L.Pereira,Neize M.Souza,Wanderley deCruz,M. QueirozBräscher,Helena M.Lima,A. Oliveiraeng2020-04-25T17:45:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:00:08.148Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
title Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
spellingShingle Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
Timm,Solange L.
title_short Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
title_full Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
title_fullStr Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
title_full_unstemmed Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
title_sort Isolation of an enriched plasma membrame subpellicular microtubule fraction of Leishmania mexicana amazonensis
author Timm,Solange L.
author_facet Timm,Solange L.
Leon,Leonor L.
Pereira,Neize M.
Souza,Wanderley de
Cruz,M. Queiroz
Bräscher,Helena M.
Lima,A. Oliveira
author_role author
author2 Leon,Leonor L.
Pereira,Neize M.
Souza,Wanderley de
Cruz,M. Queiroz
Bräscher,Helena M.
Lima,A. Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Timm,Solange L.
Leon,Leonor L.
Pereira,Neize M.
Souza,Wanderley de
Cruz,M. Queiroz
Bräscher,Helena M.
Lima,A. Oliveira
dc.description.none.fl_txt_mv A cell fractionation procedure previously developed for Trypanosoma cruzi was applied to isolated the plasma membrane of promastigotes of Leishania mexicana amazonensis. The cell, swollen in an hypotonic mediun, were disrupted in the presence of a nonionic detergent and the membrane fraction isolated by differencial centrifugation. Electron microscopy showed that the fraction consisted of pieces of the plasma membrane associated with subpellicular microtubules. It was also shown that this fraction is able to induce cell-mediated immune response in mice.
description A cell fractionation procedure previously developed for Trypanosoma cruzi was applied to isolated the plasma membrane of promastigotes of Leishania mexicana amazonensis. The cell, swollen in an hypotonic mediun, were disrupted in the presence of a nonionic detergent and the membrane fraction isolated by differencial centrifugation. Electron microscopy showed that the fraction consisted of pieces of the plasma membrane associated with subpellicular microtubules. It was also shown that this fraction is able to induce cell-mediated immune response in mice.
publishDate 1980
dc.date.none.fl_str_mv 1980-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761980000100014
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761980000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761980000100014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.75 n.1-2 1980
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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