Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100037 |
Resumo: | Cystic fibrosis (CF) patients with Burkholderia cepacia complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recAsequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recAsequencing to identify Bcc species. The recAsequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia IIIA (33.4%),Burkholderia vietnamiensis (30.6%), B. cenocepaciaIIIB (27.8%), Burkholderia multivorans (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes. |
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Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosisBurkholderia cepacia complexcystic fibrosislung diseaseGram-negative bacterial infectionDNA sequence analysesmatrix-assisted laser desorption-ionisation mass spectrometryCystic fibrosis (CF) patients with Burkholderia cepacia complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recAsequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recAsequencing to identify Bcc species. The recAsequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia IIIA (33.4%),Burkholderia vietnamiensis (30.6%), B. cenocepaciaIIIB (27.8%), Burkholderia multivorans (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes.Instituto Oswaldo Cruz, Ministério da Saúde2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100037Memórias do Instituto Oswaldo Cruz v.111 n.1 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150314info:eu-repo/semantics/openAccessVicenzi,Fernando JoséPillonetto,MarceloSouza,Helena Aguilar Peres Homem de Mello dePalmeiro,Jussara KasukoRiedi,Carlos AntônioRosario-Filho,Nelson AugustoDalla-Costa,Libera Mariaeng2020-04-25T17:52:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:53.447Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
title |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
spellingShingle |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis Vicenzi,Fernando José Burkholderia cepacia complex cystic fibrosis lung disease Gram-negative bacterial infection DNA sequence analyses matrix-assisted laser desorption-ionisation mass spectrometry |
title_short |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
title_full |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
title_fullStr |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
title_full_unstemmed |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
title_sort |
Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis |
author |
Vicenzi,Fernando José |
author_facet |
Vicenzi,Fernando José Pillonetto,Marcelo Souza,Helena Aguilar Peres Homem de Mello de Palmeiro,Jussara Kasuko Riedi,Carlos Antônio Rosario-Filho,Nelson Augusto Dalla-Costa,Libera Maria |
author_role |
author |
author2 |
Pillonetto,Marcelo Souza,Helena Aguilar Peres Homem de Mello de Palmeiro,Jussara Kasuko Riedi,Carlos Antônio Rosario-Filho,Nelson Augusto Dalla-Costa,Libera Maria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vicenzi,Fernando José Pillonetto,Marcelo Souza,Helena Aguilar Peres Homem de Mello de Palmeiro,Jussara Kasuko Riedi,Carlos Antônio Rosario-Filho,Nelson Augusto Dalla-Costa,Libera Maria |
dc.subject.por.fl_str_mv |
Burkholderia cepacia complex cystic fibrosis lung disease Gram-negative bacterial infection DNA sequence analyses matrix-assisted laser desorption-ionisation mass spectrometry |
topic |
Burkholderia cepacia complex cystic fibrosis lung disease Gram-negative bacterial infection DNA sequence analyses matrix-assisted laser desorption-ionisation mass spectrometry |
dc.description.none.fl_txt_mv |
Cystic fibrosis (CF) patients with Burkholderia cepacia complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recAsequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recAsequencing to identify Bcc species. The recAsequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia IIIA (33.4%),Burkholderia vietnamiensis (30.6%), B. cenocepaciaIIIB (27.8%), Burkholderia multivorans (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes. |
description |
Cystic fibrosis (CF) patients with Burkholderia cepacia complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recAsequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recAsequencing to identify Bcc species. The recAsequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia IIIA (33.4%),Burkholderia vietnamiensis (30.6%), B. cenocepaciaIIIB (27.8%), Burkholderia multivorans (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100037 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100037 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760150314 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.111 n.1 2016 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
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Memórias do Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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