A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)

Detalhes bibliográficos
Autor(a) principal: Campos,Jairo
Data de Publicação: 2003
Outros Autores: Andrade,Carlos Fernando S, Recco-Pimentel,Shirlei M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017
Resumo: Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.
id FIOCRUZ-4_70dd06967424bd395f00ecc858d89111
oai_identifier_str oai:scielo:S0074-02762003000300017
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)cytologymosquitovectorPolytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.Instituto Oswaldo Cruz, Ministério da Saúde2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017Memórias do Instituto Oswaldo Cruz v.98 n.3 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000300017info:eu-repo/semantics/openAccessCampos,JairoAndrade,Carlos Fernando SRecco-Pimentel,Shirlei Meng2020-04-25T17:49:03Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:54.781Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
title A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
spellingShingle A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Campos,Jairo
cytology
mosquito
vector
title_short A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
title_full A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
title_fullStr A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
title_full_unstemmed A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
title_sort A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
author Campos,Jairo
author_facet Campos,Jairo
Andrade,Carlos Fernando S
Recco-Pimentel,Shirlei M
author_role author
author2 Andrade,Carlos Fernando S
Recco-Pimentel,Shirlei M
author2_role author
author
dc.contributor.author.fl_str_mv Campos,Jairo
Andrade,Carlos Fernando S
Recco-Pimentel,Shirlei M
dc.subject.por.fl_str_mv cytology
mosquito
vector
topic cytology
mosquito
vector
dc.description.none.fl_txt_mv Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.
description Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.
publishDate 2003
dc.date.none.fl_str_mv 2003-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000300017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 n.3 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937688194580480

Registros relacionados