Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica

Detalhes bibliográficos
Autor(a) principal: Cardona-Castro,Nora
Data de Publicação: 2002
Outros Autores: Restrepo-Pineda,Eliana, Correa-Ochoa,Margarita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800016
Resumo: hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.
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spelling Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies entericahilA gene sequencesSalmonella serovars TyphiEnteritidisCholeraesuisParatyphi A and BPullorumhilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.Instituto Oswaldo Cruz, Ministério da Saúde2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800016Memórias do Instituto Oswaldo Cruz v.97 n.8 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000800016info:eu-repo/semantics/openAccessCardona-Castro,NoraRestrepo-Pineda,ElianaCorrea-Ochoa,Margaritaeng2020-04-25T17:48:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:30.211Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
title Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
spellingShingle Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
Cardona-Castro,Nora
hilA gene sequences
Salmonella serovars Typhi
Enteritidis
Choleraesuis
Paratyphi A and B
Pullorum
title_short Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
title_full Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
title_fullStr Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
title_full_unstemmed Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
title_sort Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
author Cardona-Castro,Nora
author_facet Cardona-Castro,Nora
Restrepo-Pineda,Eliana
Correa-Ochoa,Margarita
author_role author
author2 Restrepo-Pineda,Eliana
Correa-Ochoa,Margarita
author2_role author
author
dc.contributor.author.fl_str_mv Cardona-Castro,Nora
Restrepo-Pineda,Eliana
Correa-Ochoa,Margarita
dc.subject.por.fl_str_mv hilA gene sequences
Salmonella serovars Typhi
Enteritidis
Choleraesuis
Paratyphi A and B
Pullorum
topic hilA gene sequences
Salmonella serovars Typhi
Enteritidis
Choleraesuis
Paratyphi A and B
Pullorum
dc.description.none.fl_txt_mv hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.
description hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000800016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.8 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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