Infection kinetics of human adenovirus serotype 41 in HEK 293 cells

• Autor(a) principal: Siqueira-Silva,Joselma
• Data de Publicação: 2009
• Outros Autores: Yeda,Fernanda Perez, Favier,Anne-Laure, Mezin,Paulette, Silva,Misael Leonardo, Barrella,Karina Médici, Mehnert,Dolores Úrsula, Fender,Pascal, Hársi,Charlotte Marianna
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Memórias do Instituto Oswaldo Cruz
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500013
• topic: Human adenovirus F; infection kinetics; virus cultivation; confocal microscopy; electronic microscopy

The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.