Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil

Detalhes bibliográficos
Autor(a) principal: Silva,Marcio Roberto
Data de Publicação: 2013
Outros Autores: Rocha,Adalgiza da Silva, Costa,Ronaldo Rodrigues da, Alencar,Andrea Padilha de, Oliveira,Vania Maria de, Fonseca Junior,Antonio Augusto, Sales,Mariana Lazaro, Issa,Marina de Azevedo, Soares Filho,Paulo Martins, Pereira,Omara Tereza Vianello, Santos,Eduardo Calazans dos, Mendes,Rejane Silva, Ferreira,Angela Maria de Jesus, Mota,Pedro Moacyr Pinto Coelho, Suffys,Philip Noel, Guimaraes,Mark Drew Crosland
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300321
Resumo: In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.
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spelling Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of BrazilMycobacterium bovisMycobacterium tuberculosiszoonotic tuberculosis In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.Instituto Oswaldo Cruz, Ministério da Saúde2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300321Memórias do Instituto Oswaldo Cruz v.108 n.3 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762013000300010info:eu-repo/semantics/openAccessSilva,Marcio RobertoRocha,Adalgiza da SilvaCosta,Ronaldo Rodrigues daAlencar,Andrea Padilha deOliveira,Vania Maria deFonseca Junior,Antonio AugustoSales,Mariana LazaroIssa,Marina de AzevedoSoares Filho,Paulo MartinsPereira,Omara Tereza VianelloSantos,Eduardo Calazans dosMendes,Rejane SilvaFerreira,Angela Maria de JesusMota,Pedro Moacyr Pinto CoelhoSuffys,Philip NoelGuimaraes,Mark Drew Croslandeng2020-04-25T17:51:24Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:57.764Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
title Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
spellingShingle Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
Silva,Marcio Roberto
Mycobacterium bovis
Mycobacterium tuberculosis
zoonotic tuberculosis
title_short Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
title_full Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
title_fullStr Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
title_full_unstemmed Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
title_sort Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil
author Silva,Marcio Roberto
author_facet Silva,Marcio Roberto
Rocha,Adalgiza da Silva
Costa,Ronaldo Rodrigues da
Alencar,Andrea Padilha de
Oliveira,Vania Maria de
Fonseca Junior,Antonio Augusto
Sales,Mariana Lazaro
Issa,Marina de Azevedo
Soares Filho,Paulo Martins
Pereira,Omara Tereza Vianello
Santos,Eduardo Calazans dos
Mendes,Rejane Silva
Ferreira,Angela Maria de Jesus
Mota,Pedro Moacyr Pinto Coelho
Suffys,Philip Noel
Guimaraes,Mark Drew Crosland
author_role author
author2 Rocha,Adalgiza da Silva
Costa,Ronaldo Rodrigues da
Alencar,Andrea Padilha de
Oliveira,Vania Maria de
Fonseca Junior,Antonio Augusto
Sales,Mariana Lazaro
Issa,Marina de Azevedo
Soares Filho,Paulo Martins
Pereira,Omara Tereza Vianello
Santos,Eduardo Calazans dos
Mendes,Rejane Silva
Ferreira,Angela Maria de Jesus
Mota,Pedro Moacyr Pinto Coelho
Suffys,Philip Noel
Guimaraes,Mark Drew Crosland
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Marcio Roberto
Rocha,Adalgiza da Silva
Costa,Ronaldo Rodrigues da
Alencar,Andrea Padilha de
Oliveira,Vania Maria de
Fonseca Junior,Antonio Augusto
Sales,Mariana Lazaro
Issa,Marina de Azevedo
Soares Filho,Paulo Martins
Pereira,Omara Tereza Vianello
Santos,Eduardo Calazans dos
Mendes,Rejane Silva
Ferreira,Angela Maria de Jesus
Mota,Pedro Moacyr Pinto Coelho
Suffys,Philip Noel
Guimaraes,Mark Drew Crosland
dc.subject.por.fl_str_mv Mycobacterium bovis
Mycobacterium tuberculosis
zoonotic tuberculosis
topic Mycobacterium bovis
Mycobacterium tuberculosis
zoonotic tuberculosis
dc.description.none.fl_txt_mv In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.
description In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300321
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762013000300010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.3 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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