Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Detalhes bibliográficos
Autor(a) principal: Santos,Jefferson José da Silva
Data de Publicação: 2015
Outros Autores: Magalhães,Tereza, Silva Junior,José Valter Joaquim, Silva,Andréa Nazaré Monteiro Rangel da, Cordeiro,Marli Tenório, Gil,Laura Helena Vega Gonzales
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500677
Resumo: Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
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spelling Full-length infectious clone of a low passage dengue virus serotype 2 from Brazilinfectious cloneyeast homologous recombinationflavivirusFull-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.Instituto Oswaldo Cruz, Ministério da Saúde2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500677Memórias do Instituto Oswaldo Cruz v.110 n.5 2015reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150053info:eu-repo/semantics/openAccessSantos,Jefferson José da SilvaMagalhães,TerezaSilva Junior,José Valter JoaquimSilva,Andréa Nazaré Monteiro Rangel daCordeiro,Marli TenórioGil,Laura Helena Vega Gonzaleseng2020-04-25T17:52:14Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:49.136Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
spellingShingle Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
Santos,Jefferson José da Silva
infectious clone
yeast homologous recombination
flavivirus
title_short Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_full Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_fullStr Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_full_unstemmed Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_sort Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
author Santos,Jefferson José da Silva
author_facet Santos,Jefferson José da Silva
Magalhães,Tereza
Silva Junior,José Valter Joaquim
Silva,Andréa Nazaré Monteiro Rangel da
Cordeiro,Marli Tenório
Gil,Laura Helena Vega Gonzales
author_role author
author2 Magalhães,Tereza
Silva Junior,José Valter Joaquim
Silva,Andréa Nazaré Monteiro Rangel da
Cordeiro,Marli Tenório
Gil,Laura Helena Vega Gonzales
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos,Jefferson José da Silva
Magalhães,Tereza
Silva Junior,José Valter Joaquim
Silva,Andréa Nazaré Monteiro Rangel da
Cordeiro,Marli Tenório
Gil,Laura Helena Vega Gonzales
dc.subject.por.fl_str_mv infectious clone
yeast homologous recombination
flavivirus
topic infectious clone
yeast homologous recombination
flavivirus
dc.description.none.fl_txt_mv Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
description Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500677
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500677
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760150053
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.110 n.5 2015
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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