Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference

Detalhes bibliográficos
Autor(a) principal: García,Liliana Torcoroma
Data de Publicação: 2007
Outros Autores: Leite,Ney Ribeiro, Alfonzo,Juan D, Thiemann,Otavio Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600016
Resumo: The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei: TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.
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spelling Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interferencetryptophanyl tRNA synthetaseRNA interferencekinetoplastidTrypanosoma bruceThe kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei: TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.Instituto Oswaldo Cruz, Ministério da Saúde2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600016Memórias do Instituto Oswaldo Cruz v.102 n.6 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000090info:eu-repo/semantics/openAccessGarcía,Liliana TorcoromaLeite,Ney RibeiroAlfonzo,Juan DThiemann,Otavio Henriqueeng2020-04-25T17:50:11Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:18.961Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
title Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
spellingShingle Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
García,Liliana Torcoroma
tryptophanyl tRNA synthetase
RNA interference
kinetoplastid
Trypanosoma bruce
title_short Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
title_full Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
title_fullStr Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
title_full_unstemmed Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
title_sort Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
author García,Liliana Torcoroma
author_facet García,Liliana Torcoroma
Leite,Ney Ribeiro
Alfonzo,Juan D
Thiemann,Otavio Henrique
author_role author
author2 Leite,Ney Ribeiro
Alfonzo,Juan D
Thiemann,Otavio Henrique
author2_role author
author
author
dc.contributor.author.fl_str_mv García,Liliana Torcoroma
Leite,Ney Ribeiro
Alfonzo,Juan D
Thiemann,Otavio Henrique
dc.subject.por.fl_str_mv tryptophanyl tRNA synthetase
RNA interference
kinetoplastid
Trypanosoma bruce
topic tryptophanyl tRNA synthetase
RNA interference
kinetoplastid
Trypanosoma bruce
dc.description.none.fl_txt_mv The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei: TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.
description The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei: TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762007005000090
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.102 n.6 2007
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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