Further studies on the molecular systematics of Biomphalaria snails from Brazil

Detalhes bibliográficos
Autor(a) principal: Vidigal,Teofânia HDA
Data de Publicação: 2000
Outros Autores: Caldeira,Roberta Lima, Simpson,Andrew JG, Carvalho,Omar S
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009
Resumo: The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snails
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spelling Further studies on the molecular systematics of Biomphalaria snails from BrazilBiomphalariaribosomal DNAinternal transcribed spacerpolymerase chain reactionBrazilThe polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snailsInstituto Oswaldo Cruz, Ministério da Saúde2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009Memórias do Instituto Oswaldo Cruz v.95 n.1 2000reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762000000100009info:eu-repo/semantics/openAccessVidigal,Teofânia HDACaldeira,Roberta LimaSimpson,Andrew JGCarvalho,Omar Seng2020-04-25T17:48:20Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:09:30.529Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Further studies on the molecular systematics of Biomphalaria snails from Brazil
title Further studies on the molecular systematics of Biomphalaria snails from Brazil
spellingShingle Further studies on the molecular systematics of Biomphalaria snails from Brazil
Vidigal,Teofânia HDA
Biomphalaria
ribosomal DNA
internal transcribed spacer
polymerase chain reaction
Brazil
title_short Further studies on the molecular systematics of Biomphalaria snails from Brazil
title_full Further studies on the molecular systematics of Biomphalaria snails from Brazil
title_fullStr Further studies on the molecular systematics of Biomphalaria snails from Brazil
title_full_unstemmed Further studies on the molecular systematics of Biomphalaria snails from Brazil
title_sort Further studies on the molecular systematics of Biomphalaria snails from Brazil
author Vidigal,Teofânia HDA
author_facet Vidigal,Teofânia HDA
Caldeira,Roberta Lima
Simpson,Andrew JG
Carvalho,Omar S
author_role author
author2 Caldeira,Roberta Lima
Simpson,Andrew JG
Carvalho,Omar S
author2_role author
author
author
dc.contributor.author.fl_str_mv Vidigal,Teofânia HDA
Caldeira,Roberta Lima
Simpson,Andrew JG
Carvalho,Omar S
dc.subject.por.fl_str_mv Biomphalaria
ribosomal DNA
internal transcribed spacer
polymerase chain reaction
Brazil
topic Biomphalaria
ribosomal DNA
internal transcribed spacer
polymerase chain reaction
Brazil
dc.description.none.fl_txt_mv The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snails
description The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snails
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762000000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.95 n.1 2000
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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