Low impact to fixed cell processing aiming transmission electron microscopy

Detalhes bibliográficos
Autor(a) principal: Barth,Ortrud Monika
Data de Publicação: 2016
Outros Autores: Silva,Marcos Alexandre Nunes da, Barreto-Vieira,Debora Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600411
Resumo: In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.
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spelling Low impact to fixed cell processing aiming transmission electron microscopycell fixationtransmission electron microscopylow impactDENVIn cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.Instituto Oswaldo Cruz, Ministério da Saúde2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600411Memórias do Instituto Oswaldo Cruz v.111 n.6 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150433info:eu-repo/semantics/openAccessBarth,Ortrud MonikaSilva,Marcos Alexandre Nunes daBarreto-Vieira,Debora Ferreiraeng2020-04-25T17:52:27Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:24.87Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Low impact to fixed cell processing aiming transmission electron microscopy
title Low impact to fixed cell processing aiming transmission electron microscopy
spellingShingle Low impact to fixed cell processing aiming transmission electron microscopy
Barth,Ortrud Monika
cell fixation
transmission electron microscopy
low impact
DENV
title_short Low impact to fixed cell processing aiming transmission electron microscopy
title_full Low impact to fixed cell processing aiming transmission electron microscopy
title_fullStr Low impact to fixed cell processing aiming transmission electron microscopy
title_full_unstemmed Low impact to fixed cell processing aiming transmission electron microscopy
title_sort Low impact to fixed cell processing aiming transmission electron microscopy
author Barth,Ortrud Monika
author_facet Barth,Ortrud Monika
Silva,Marcos Alexandre Nunes da
Barreto-Vieira,Debora Ferreira
author_role author
author2 Silva,Marcos Alexandre Nunes da
Barreto-Vieira,Debora Ferreira
author2_role author
author
dc.contributor.author.fl_str_mv Barth,Ortrud Monika
Silva,Marcos Alexandre Nunes da
Barreto-Vieira,Debora Ferreira
dc.subject.por.fl_str_mv cell fixation
transmission electron microscopy
low impact
DENV
topic cell fixation
transmission electron microscopy
low impact
DENV
dc.description.none.fl_txt_mv In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.
description In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600411
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600411
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760150433
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.111 n.6 2016
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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