A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus

• Autor(a) principal: Tolardo,Aline Lavado
• Data de Publicação: 2016
• Outros Autores: Souza,William Marciel de, Romeiro,Marilia Farignoli, Vieira,Luiz Carlos, Luna,Luciano Kleber de Souza, Henriques,Dyana Alves, Araujo,Jansen de, Siqueira,Carlos Eduardo Hassegawa, Colombo,Tatiana Elias, Aquino,Victor Hugo, Fonseca,Benedito Antonio Lopes da, Bronzoni,Roberta Vieira de Morais, Nogueira,Maurício Lacerda, Durigon,Edison Luiz, Figueiredo,Luiz Tadeu Moraes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Memórias do Instituto Oswaldo Cruz
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600385
• topic: Vesiculovirus; quantitative real-time RT-PCR; diagnosis of vesicular stomatitis; zoonotic virus

Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and implementation of strategies for the containment of virus spread. We show here a sensitive and reproducible real-time reverse transcriptase polymerase chain reaction (RT-PCR) for detection and quantification of VSV. The assay was evaluated with arthropods and serum samples obtained from horses, cattle and patients with acute febrile disease. The real-time RT-PCR amplified the Piry, Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature 81.02 ± 0.8ºC, and the sensitivity of assay was estimated in 10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been detected in samples of horses and cattle, but not detected in human sera or arthropods. Thus, this assay allows a preliminary differential diagnosis of VSV infections.