Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900058 |
Resumo: | The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni. |
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Memórias do Instituto Oswaldo Cruz |
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Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoniSchistosoma mansoniimmunodiagnosissynthetic peptidesThe immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.Instituto Oswaldo Cruz, Ministério da Saúde2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900058Memórias do Instituto Oswaldo Cruz v.101 suppl.1 2006reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762006000900058info:eu-repo/semantics/openAccessOliveira,Edward José deKanamura,Hermínia YohkoTakei,KiokoHirata,Rosario Dominguez CrespoNguyen,Nga YenHirata,Mário Hiroyukieng2020-04-25T17:49:44Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:14:21.547Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
title |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
spellingShingle |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni Oliveira,Edward José de Schistosoma mansoni immunodiagnosis synthetic peptides |
title_short |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
title_full |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
title_fullStr |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
title_full_unstemmed |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
title_sort |
Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni |
author |
Oliveira,Edward José de |
author_facet |
Oliveira,Edward José de Kanamura,Hermínia Yohko Takei,Kioko Hirata,Rosario Dominguez Crespo Nguyen,Nga Yen Hirata,Mário Hiroyuki |
author_role |
author |
author2 |
Kanamura,Hermínia Yohko Takei,Kioko Hirata,Rosario Dominguez Crespo Nguyen,Nga Yen Hirata,Mário Hiroyuki |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Edward José de Kanamura,Hermínia Yohko Takei,Kioko Hirata,Rosario Dominguez Crespo Nguyen,Nga Yen Hirata,Mário Hiroyuki |
dc.subject.por.fl_str_mv |
Schistosoma mansoni immunodiagnosis synthetic peptides |
topic |
Schistosoma mansoni immunodiagnosis synthetic peptides |
dc.description.none.fl_txt_mv |
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni. |
description |
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900058 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900058 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762006000900058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.101 suppl.1 2006 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937698447556608 |