Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni

• Autor(a) principal: Oliveira,Edward José de
• Data de Publicação: 2006
• Outros Autores: Kanamura,Hermínia Yohko, Takei,Kioko, Hirata,Rosario Dominguez Crespo, Nguyen,Nga Yen, Hirata,Mário Hiroyuki
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Memórias do Instituto Oswaldo Cruz
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900058
• topic: Schistosoma mansoni; immunodiagnosis; synthetic peptides

The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.