Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species

Detalhes bibliográficos
Autor(a) principal: Rosa,EAR
Data de Publicação: 2000
Outros Autores: Rosa,RT, Pereira,CV, Boriollo,MFG, Höfling,JF
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600009
Resumo: Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.
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spelling Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida speciespolyacrylamide gel electrophoresismultilocus enzyme electrophoresisCandidanumerical analysisElectrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.Instituto Oswaldo Cruz, Ministério da Saúde2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600009Memórias do Instituto Oswaldo Cruz v.95 n.6 2000reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762000000600009info:eu-repo/semantics/openAccessRosa,EARRosa,RTPereira,CVBoriollo,MFGHöfling,JFeng2020-04-25T17:48:26Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:09:53.626Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
title Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
spellingShingle Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
Rosa,EAR
polyacrylamide gel electrophoresis
multilocus enzyme electrophoresis
Candida
numerical analysis
title_short Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
title_full Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
title_fullStr Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
title_full_unstemmed Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
title_sort Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
author Rosa,EAR
author_facet Rosa,EAR
Rosa,RT
Pereira,CV
Boriollo,MFG
Höfling,JF
author_role author
author2 Rosa,RT
Pereira,CV
Boriollo,MFG
Höfling,JF
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rosa,EAR
Rosa,RT
Pereira,CV
Boriollo,MFG
Höfling,JF
dc.subject.por.fl_str_mv polyacrylamide gel electrophoresis
multilocus enzyme electrophoresis
Candida
numerical analysis
topic polyacrylamide gel electrophoresis
multilocus enzyme electrophoresis
Candida
numerical analysis
dc.description.none.fl_txt_mv Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.
description Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762000000600009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.95 n.6 2000
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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