Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
Autor(a) principal: | |
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Data de Publicação: | 1990 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003 |
Resumo: | Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths. |
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Memórias do Instituto Oswaldo Cruz |
spelling |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serumnylon wool columnshuman monokine productionthymocyte costimulatory assayLPSperipheral blood monocytesDiverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths.Instituto Oswaldo Cruz, Ministério da Saúde1990-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003Memórias do Instituto Oswaldo Cruz v.85 n.2 1990reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761990000200003info:eu-repo/semantics/openAccessDalmau,S. R.Freitas,C. S.eng2020-04-25T17:46:28Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:03:42.684Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
title |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
spellingShingle |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum Dalmau,S. R. nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes |
title_short |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
title_full |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
title_fullStr |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
title_full_unstemmed |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
title_sort |
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
author |
Dalmau,S. R. |
author_facet |
Dalmau,S. R. Freitas,C. S. |
author_role |
author |
author2 |
Freitas,C. S. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Dalmau,S. R. Freitas,C. S. |
dc.subject.por.fl_str_mv |
nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes |
topic |
nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes |
dc.description.none.fl_txt_mv |
Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths. |
description |
Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths. |
publishDate |
1990 |
dc.date.none.fl_str_mv |
1990-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761990000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.85 n.2 1990 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937655554506752 |