In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury

Detalhes bibliográficos
Autor(a) principal: Souza,Elen Mello de
Data de Publicação: 2004
Outros Autores: Henriques-Pons,Andrea, Bailly,Christian, Lansiaux,Amelie, Araújo-Jorge,Tânia Cremonine, Soeiro,Maria de Nazaré Correia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700006
Resumo: Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
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spelling In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injurycreatine kinasein vitro assayscardiac damagePrimary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.Instituto Oswaldo Cruz, Ministério da Saúde2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700006Memórias do Instituto Oswaldo Cruz v.99 n.7 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000700006info:eu-repo/semantics/openAccessSouza,Elen Mello deHenriques-Pons,AndreaBailly,ChristianLansiaux,AmelieAraújo-Jorge,Tânia CremonineSoeiro,Maria de Nazaré Correiaeng2020-04-25T17:49:18Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:48.178Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
title In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
spellingShingle In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
Souza,Elen Mello de
creatine kinase
in vitro assays
cardiac damage
title_short In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
title_full In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
title_fullStr In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
title_full_unstemmed In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
title_sort In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
author Souza,Elen Mello de
author_facet Souza,Elen Mello de
Henriques-Pons,Andrea
Bailly,Christian
Lansiaux,Amelie
Araújo-Jorge,Tânia Cremonine
Soeiro,Maria de Nazaré Correia
author_role author
author2 Henriques-Pons,Andrea
Bailly,Christian
Lansiaux,Amelie
Araújo-Jorge,Tânia Cremonine
Soeiro,Maria de Nazaré Correia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza,Elen Mello de
Henriques-Pons,Andrea
Bailly,Christian
Lansiaux,Amelie
Araújo-Jorge,Tânia Cremonine
Soeiro,Maria de Nazaré Correia
dc.subject.por.fl_str_mv creatine kinase
in vitro assays
cardiac damage
topic creatine kinase
in vitro assays
cardiac damage
dc.description.none.fl_txt_mv Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
description Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
publishDate 2004
dc.date.none.fl_str_mv 2004-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700006
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000700006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 n.7 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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