DsRed2 transient expression in Culex quinquefasciatus mosquitoes

Detalhes bibliográficos
Autor(a) principal: Wilke,Andre Barretto Bruno
Data de Publicação: 2013
Outros Autores: Scaife,Sarah, Alphey,Luke, Marrelli,Mauro Toledo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400529
Resumo: Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
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spelling DsRed2 transient expression in Culex quinquefasciatus mosquitoesCulex quinquefasciatustransient expressionDsRed2 Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.Instituto Oswaldo Cruz, Ministério da Saúde2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400529Memórias do Instituto Oswaldo Cruz v.108 n.4 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762013000400023info:eu-repo/semantics/openAccessWilke,Andre Barretto BrunoScaife,SarahAlphey,LukeMarrelli,Mauro Toledoeng2020-04-25T17:51:32Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:04.384Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv DsRed2 transient expression in Culex quinquefasciatus mosquitoes
title DsRed2 transient expression in Culex quinquefasciatus mosquitoes
spellingShingle DsRed2 transient expression in Culex quinquefasciatus mosquitoes
Wilke,Andre Barretto Bruno
Culex quinquefasciatus
transient expression
DsRed2
title_short DsRed2 transient expression in Culex quinquefasciatus mosquitoes
title_full DsRed2 transient expression in Culex quinquefasciatus mosquitoes
title_fullStr DsRed2 transient expression in Culex quinquefasciatus mosquitoes
title_full_unstemmed DsRed2 transient expression in Culex quinquefasciatus mosquitoes
title_sort DsRed2 transient expression in Culex quinquefasciatus mosquitoes
author Wilke,Andre Barretto Bruno
author_facet Wilke,Andre Barretto Bruno
Scaife,Sarah
Alphey,Luke
Marrelli,Mauro Toledo
author_role author
author2 Scaife,Sarah
Alphey,Luke
Marrelli,Mauro Toledo
author2_role author
author
author
dc.contributor.author.fl_str_mv Wilke,Andre Barretto Bruno
Scaife,Sarah
Alphey,Luke
Marrelli,Mauro Toledo
dc.subject.por.fl_str_mv Culex quinquefasciatus
transient expression
DsRed2
topic Culex quinquefasciatus
transient expression
DsRed2
dc.description.none.fl_txt_mv Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
description Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400529
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400529
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762013000400023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.4 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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