2D-immunoblotting analysis of Sporothrix schenckii cell wall

Detalhes bibliográficos
Autor(a) principal: Ruiz-Baca,Estela
Data de Publicação: 2011
Outros Autores: Mora-Montes,Héctor M, López-Romero,Everardo, Toriello,Conchita, Mojica-Marín,Virgilio, Urtiz-Estrada,Norma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200021
Resumo: We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
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spelling 2D-immunoblotting analysis of Sporothrix schenckii cell wallsporotrichosisSporothrix schenckiicell wallglycoproteins2D-immunoblotting analysisWe utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.Instituto Oswaldo Cruz, Ministério da Saúde2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200021Memórias do Instituto Oswaldo Cruz v.106 n.2 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000200021info:eu-repo/semantics/openAccessRuiz-Baca,EstelaMora-Montes,Héctor MLópez-Romero,EverardoToriello,ConchitaMojica-Marín,VirgilioUrtiz-Estrada,Normaeng2020-04-25T17:50:59Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:33.0Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv 2D-immunoblotting analysis of Sporothrix schenckii cell wall
title 2D-immunoblotting analysis of Sporothrix schenckii cell wall
spellingShingle 2D-immunoblotting analysis of Sporothrix schenckii cell wall
Ruiz-Baca,Estela
sporotrichosis
Sporothrix schenckii
cell wall
glycoproteins
2D-immunoblotting analysis
title_short 2D-immunoblotting analysis of Sporothrix schenckii cell wall
title_full 2D-immunoblotting analysis of Sporothrix schenckii cell wall
title_fullStr 2D-immunoblotting analysis of Sporothrix schenckii cell wall
title_full_unstemmed 2D-immunoblotting analysis of Sporothrix schenckii cell wall
title_sort 2D-immunoblotting analysis of Sporothrix schenckii cell wall
author Ruiz-Baca,Estela
author_facet Ruiz-Baca,Estela
Mora-Montes,Héctor M
López-Romero,Everardo
Toriello,Conchita
Mojica-Marín,Virgilio
Urtiz-Estrada,Norma
author_role author
author2 Mora-Montes,Héctor M
López-Romero,Everardo
Toriello,Conchita
Mojica-Marín,Virgilio
Urtiz-Estrada,Norma
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ruiz-Baca,Estela
Mora-Montes,Héctor M
López-Romero,Everardo
Toriello,Conchita
Mojica-Marín,Virgilio
Urtiz-Estrada,Norma
dc.subject.por.fl_str_mv sporotrichosis
Sporothrix schenckii
cell wall
glycoproteins
2D-immunoblotting analysis
topic sporotrichosis
Sporothrix schenckii
cell wall
glycoproteins
2D-immunoblotting analysis
dc.description.none.fl_txt_mv We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
description We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200021
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000200021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.2 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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