Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Detalhes bibliográficos
Autor(a) principal: Illnait,Ma.Teresa
Data de Publicação: 2001
Outros Autores: Vilaseca,Juan C, Fernández,Carlos M, Martínez,Gerardo F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018
Resumo: An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
id FIOCRUZ-4_be1be15e3071e71b640a3368144ac71b
oai_identifier_str oai:scielo:S0074-02762001000200018
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigencryptococcal antigenantigen quantificationantigen detectionenzyme-linked immunosorbent assay-ELISAAn enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.Instituto Oswaldo Cruz, Ministério da Saúde2001-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018Memórias do Instituto Oswaldo Cruz v.96 n.2 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000200018info:eu-repo/semantics/openAccessIllnait,Ma.TeresaVilaseca,Juan CFernández,Carlos MMartínez,Gerardo Feng2020-04-25T17:48:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:11.461Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
spellingShingle Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
Illnait,Ma.Teresa
cryptococcal antigen
antigen quantification
antigen detection
enzyme-linked immunosorbent assay-ELISA
title_short Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_full Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_fullStr Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_full_unstemmed Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_sort Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
author Illnait,Ma.Teresa
author_facet Illnait,Ma.Teresa
Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
author_role author
author2 Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
author2_role author
author
author
dc.contributor.author.fl_str_mv Illnait,Ma.Teresa
Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
dc.subject.por.fl_str_mv cryptococcal antigen
antigen quantification
antigen detection
enzyme-linked immunosorbent assay-ELISA
topic cryptococcal antigen
antigen quantification
antigen detection
enzyme-linked immunosorbent assay-ELISA
dc.description.none.fl_txt_mv An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
description An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
publishDate 2001
dc.date.none.fl_str_mv 2001-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000200018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 n.2 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937681065312256

Registros relacionados