Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Lane,Joshua E
Data de Publicação: 2003
Outros Autores: Ribeiro-Rodrigues,Rodrigo, Olivares-Villagómez,Danyvid, Vnencak-Jones,Cindy L, McCurley,Thomas L, Carter,Clint E
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300013
Resumo: The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.
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spelling Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reactionin situ polymerase chain reaction (PCR)indirect in situ PCRdirect in situ PCRTrypanosoma cruzimurine cardiac tissueThe use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.Instituto Oswaldo Cruz, Ministério da Saúde2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300013Memórias do Instituto Oswaldo Cruz v.98 n.3 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000300013info:eu-repo/semantics/openAccessLane,Joshua ERibeiro-Rodrigues,RodrigoOlivares-Villagómez,DanyvidVnencak-Jones,Cindy LMcCurley,Thomas LCarter,Clint Eeng2020-04-25T17:49:03Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:54.013Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
title Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
spellingShingle Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
Lane,Joshua E
in situ polymerase chain reaction (PCR)
indirect in situ PCR
direct in situ PCR
Trypanosoma cruzi
murine cardiac tissue
title_short Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
title_full Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
title_fullStr Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
title_full_unstemmed Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
title_sort Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
author Lane,Joshua E
author_facet Lane,Joshua E
Ribeiro-Rodrigues,Rodrigo
Olivares-Villagómez,Danyvid
Vnencak-Jones,Cindy L
McCurley,Thomas L
Carter,Clint E
author_role author
author2 Ribeiro-Rodrigues,Rodrigo
Olivares-Villagómez,Danyvid
Vnencak-Jones,Cindy L
McCurley,Thomas L
Carter,Clint E
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lane,Joshua E
Ribeiro-Rodrigues,Rodrigo
Olivares-Villagómez,Danyvid
Vnencak-Jones,Cindy L
McCurley,Thomas L
Carter,Clint E
dc.subject.por.fl_str_mv in situ polymerase chain reaction (PCR)
indirect in situ PCR
direct in situ PCR
Trypanosoma cruzi
murine cardiac tissue
topic in situ polymerase chain reaction (PCR)
indirect in situ PCR
direct in situ PCR
Trypanosoma cruzi
murine cardiac tissue
dc.description.none.fl_txt_mv The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.
description The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.
publishDate 2003
dc.date.none.fl_str_mv 2003-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000300013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 n.3 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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