Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998

Detalhes bibliográficos
Autor(a) principal: Chimara,Erica
Data de Publicação: 2004
Outros Autores: Giampaglia,Carmen Maria Saraiva, Martins,Maria Conceição, Telles,Maria Alice da Silva, Ueki,Suely Yoko Mizuka, Ferrazoli,Lucilaine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700013
Resumo: Mycobacterium kansasii is the most common cause of pulmonary nontuberculous mycobacteria infection and classical identification of this pathogen needs a time consuming phenotypic tests. Polymerase chain reaction-restriction fragment lenght polymorphism analysis (PRA) of the gene enconding for the 65kDa heat shock (hsp65) protein offers an easy, rapid, and inexpensive procedure to identify and subtype M. kansasii isolates. In the present study, we performed a retrospective analysis of patients who had mycobacteria identified on the basis of phenotypic tests by means of a review of database at Mycobacteria Laboratory of the Instituto Adolfo Lutz in the period 1995-1998. A total of 9381 clinical isolates were analyzed of which 7777 (82.9%) were identified as M. tuberculosis complex and 1604 (17.1%) as nontuberculous mycobacteria. Of the 296 M. kansasii isolates, 189 (63.8%) isolates obtained from 119 patients were viable and were analyzed by PRA-hsp65. Hundred eight two (98.9%) were classified as M. kansasii type I. Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of São Paulo was almost exclusively subtype I regardless of HIV status.
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spelling Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998Mycobacterium kansasiinontuberculous mycobacteriahsp65restriction analysisMycobacterium kansasii is the most common cause of pulmonary nontuberculous mycobacteria infection and classical identification of this pathogen needs a time consuming phenotypic tests. Polymerase chain reaction-restriction fragment lenght polymorphism analysis (PRA) of the gene enconding for the 65kDa heat shock (hsp65) protein offers an easy, rapid, and inexpensive procedure to identify and subtype M. kansasii isolates. In the present study, we performed a retrospective analysis of patients who had mycobacteria identified on the basis of phenotypic tests by means of a review of database at Mycobacteria Laboratory of the Instituto Adolfo Lutz in the period 1995-1998. A total of 9381 clinical isolates were analyzed of which 7777 (82.9%) were identified as M. tuberculosis complex and 1604 (17.1%) as nontuberculous mycobacteria. Of the 296 M. kansasii isolates, 189 (63.8%) isolates obtained from 119 patients were viable and were analyzed by PRA-hsp65. Hundred eight two (98.9%) were classified as M. kansasii type I. Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of São Paulo was almost exclusively subtype I regardless of HIV status.Instituto Oswaldo Cruz, Ministério da Saúde2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700013Memórias do Instituto Oswaldo Cruz v.99 n.7 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000700013info:eu-repo/semantics/openAccessChimara,EricaGiampaglia,Carmen Maria SaraivaMartins,Maria ConceiçãoTelles,Maria Alice da SilvaUeki,Suely Yoko MizukaFerrazoli,Lucilaineeng2020-04-25T17:49:20Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:49.229Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
title Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
spellingShingle Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
Chimara,Erica
Mycobacterium kansasii
nontuberculous mycobacteria
hsp65
restriction analysis
title_short Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
title_full Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
title_fullStr Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
title_full_unstemmed Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
title_sort Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
author Chimara,Erica
author_facet Chimara,Erica
Giampaglia,Carmen Maria Saraiva
Martins,Maria Conceição
Telles,Maria Alice da Silva
Ueki,Suely Yoko Mizuka
Ferrazoli,Lucilaine
author_role author
author2 Giampaglia,Carmen Maria Saraiva
Martins,Maria Conceição
Telles,Maria Alice da Silva
Ueki,Suely Yoko Mizuka
Ferrazoli,Lucilaine
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chimara,Erica
Giampaglia,Carmen Maria Saraiva
Martins,Maria Conceição
Telles,Maria Alice da Silva
Ueki,Suely Yoko Mizuka
Ferrazoli,Lucilaine
dc.subject.por.fl_str_mv Mycobacterium kansasii
nontuberculous mycobacteria
hsp65
restriction analysis
topic Mycobacterium kansasii
nontuberculous mycobacteria
hsp65
restriction analysis
dc.description.none.fl_txt_mv Mycobacterium kansasii is the most common cause of pulmonary nontuberculous mycobacteria infection and classical identification of this pathogen needs a time consuming phenotypic tests. Polymerase chain reaction-restriction fragment lenght polymorphism analysis (PRA) of the gene enconding for the 65kDa heat shock (hsp65) protein offers an easy, rapid, and inexpensive procedure to identify and subtype M. kansasii isolates. In the present study, we performed a retrospective analysis of patients who had mycobacteria identified on the basis of phenotypic tests by means of a review of database at Mycobacteria Laboratory of the Instituto Adolfo Lutz in the period 1995-1998. A total of 9381 clinical isolates were analyzed of which 7777 (82.9%) were identified as M. tuberculosis complex and 1604 (17.1%) as nontuberculous mycobacteria. Of the 296 M. kansasii isolates, 189 (63.8%) isolates obtained from 119 patients were viable and were analyzed by PRA-hsp65. Hundred eight two (98.9%) were classified as M. kansasii type I. Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of São Paulo was almost exclusively subtype I regardless of HIV status.
description Mycobacterium kansasii is the most common cause of pulmonary nontuberculous mycobacteria infection and classical identification of this pathogen needs a time consuming phenotypic tests. Polymerase chain reaction-restriction fragment lenght polymorphism analysis (PRA) of the gene enconding for the 65kDa heat shock (hsp65) protein offers an easy, rapid, and inexpensive procedure to identify and subtype M. kansasii isolates. In the present study, we performed a retrospective analysis of patients who had mycobacteria identified on the basis of phenotypic tests by means of a review of database at Mycobacteria Laboratory of the Instituto Adolfo Lutz in the period 1995-1998. A total of 9381 clinical isolates were analyzed of which 7777 (82.9%) were identified as M. tuberculosis complex and 1604 (17.1%) as nontuberculous mycobacteria. Of the 296 M. kansasii isolates, 189 (63.8%) isolates obtained from 119 patients were viable and were analyzed by PRA-hsp65. Hundred eight two (98.9%) were classified as M. kansasii type I. Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of São Paulo was almost exclusively subtype I regardless of HIV status.
publishDate 2004
dc.date.none.fl_str_mv 2004-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000700013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 n.7 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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reponame_str Memórias do Instituto Oswaldo Cruz
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instname_str Fundação Oswaldo Cruz
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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