Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Vieira,Carmen Baur
Data de Publicação: 2012
Outros Autores: Mendes,Anna Carolina de Oliveira, Guimarães,Flávia Ramos, Fumian,Tulio Machado, Leite,José Paulo Gagliardi, Gaspar,Ana Maria Coimbra, Miagostovich,Marize Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600012
Resumo: The aim of this study was to determine the occurrences of the group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in the surface waters of an urban lagoon (Rodrigo de Freitas Lagoon) in the city of Rio de Janeiro, Brazil. During one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). The samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and quantitative polymerase chain reaction assays. RVA was the most prevalent virus detected (24.3%) with a viral load ranging from 3.0 x 10¹-5.6 x 10(4) genome copies/L, followed by NoV (18.8%) and HAdV (16.7%). Considering water samples suitable for bathing, according to Escherichia coli criterion (< 2,000 most probable number/100 mL), viruses were detected in 50% (57/114) of them. Physicochemical parameters were also measured and showed possible correlations between turbidity and RVA presence and between pH and NoV presence. These data demonstrate the importance of considering viral parameters to ensure water quality and the utilisation of these parameters as additional tools for the characterisation of environmental contamination.
id FIOCRUZ-4_ce8c61ff4bccfc7eff2da02db8a08e7b
oai_identifier_str oai:scielo:S0074-02762012000600012
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazilgroup A rotavirusnorovirusadenovirusRodrigo de Freitas Lagoonrecreational waterThe aim of this study was to determine the occurrences of the group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in the surface waters of an urban lagoon (Rodrigo de Freitas Lagoon) in the city of Rio de Janeiro, Brazil. During one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). The samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and quantitative polymerase chain reaction assays. RVA was the most prevalent virus detected (24.3%) with a viral load ranging from 3.0 x 10¹-5.6 x 10(4) genome copies/L, followed by NoV (18.8%) and HAdV (16.7%). Considering water samples suitable for bathing, according to Escherichia coli criterion (< 2,000 most probable number/100 mL), viruses were detected in 50% (57/114) of them. Physicochemical parameters were also measured and showed possible correlations between turbidity and RVA presence and between pH and NoV presence. These data demonstrate the importance of considering viral parameters to ensure water quality and the utilisation of these parameters as additional tools for the characterisation of environmental contamination.Instituto Oswaldo Cruz, Ministério da Saúde2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600012Memórias do Instituto Oswaldo Cruz v.107 n.6 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000600012info:eu-repo/semantics/openAccessVieira,Carmen BaurMendes,Anna Carolina de OliveiraGuimarães,Flávia RamosFumian,Tulio MachadoLeite,José Paulo GagliardiGaspar,Ana Maria CoimbraMiagostovich,Marize Pereiraeng2020-04-25T17:51:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:30.442Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
title Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
spellingShingle Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
Vieira,Carmen Baur
group A rotavirus
norovirus
adenovirus
Rodrigo de Freitas Lagoon
recreational water
title_short Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
title_full Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
title_fullStr Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
title_full_unstemmed Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
title_sort Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil
author Vieira,Carmen Baur
author_facet Vieira,Carmen Baur
Mendes,Anna Carolina de Oliveira
Guimarães,Flávia Ramos
Fumian,Tulio Machado
Leite,José Paulo Gagliardi
Gaspar,Ana Maria Coimbra
Miagostovich,Marize Pereira
author_role author
author2 Mendes,Anna Carolina de Oliveira
Guimarães,Flávia Ramos
Fumian,Tulio Machado
Leite,José Paulo Gagliardi
Gaspar,Ana Maria Coimbra
Miagostovich,Marize Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira,Carmen Baur
Mendes,Anna Carolina de Oliveira
Guimarães,Flávia Ramos
Fumian,Tulio Machado
Leite,José Paulo Gagliardi
Gaspar,Ana Maria Coimbra
Miagostovich,Marize Pereira
dc.subject.por.fl_str_mv group A rotavirus
norovirus
adenovirus
Rodrigo de Freitas Lagoon
recreational water
topic group A rotavirus
norovirus
adenovirus
Rodrigo de Freitas Lagoon
recreational water
dc.description.none.fl_txt_mv The aim of this study was to determine the occurrences of the group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in the surface waters of an urban lagoon (Rodrigo de Freitas Lagoon) in the city of Rio de Janeiro, Brazil. During one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). The samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and quantitative polymerase chain reaction assays. RVA was the most prevalent virus detected (24.3%) with a viral load ranging from 3.0 x 10¹-5.6 x 10(4) genome copies/L, followed by NoV (18.8%) and HAdV (16.7%). Considering water samples suitable for bathing, according to Escherichia coli criterion (< 2,000 most probable number/100 mL), viruses were detected in 50% (57/114) of them. Physicochemical parameters were also measured and showed possible correlations between turbidity and RVA presence and between pH and NoV presence. These data demonstrate the importance of considering viral parameters to ensure water quality and the utilisation of these parameters as additional tools for the characterisation of environmental contamination.
description The aim of this study was to determine the occurrences of the group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in the surface waters of an urban lagoon (Rodrigo de Freitas Lagoon) in the city of Rio de Janeiro, Brazil. During one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). The samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and quantitative polymerase chain reaction assays. RVA was the most prevalent virus detected (24.3%) with a viral load ranging from 3.0 x 10¹-5.6 x 10(4) genome copies/L, followed by NoV (18.8%) and HAdV (16.7%). Considering water samples suitable for bathing, according to Escherichia coli criterion (< 2,000 most probable number/100 mL), viruses were detected in 50% (57/114) of them. Physicochemical parameters were also measured and showed possible correlations between turbidity and RVA presence and between pH and NoV presence. These data demonstrate the importance of considering viral parameters to ensure water quality and the utilisation of these parameters as additional tools for the characterisation of environmental contamination.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600012
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000600012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.6 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937712134619136

Registros relacionados