The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200009 |
Resumo: | Several studies conducted all over the world have reported that the influenza virus is associated with great morbidity and mortality rates. In this study, we analyzed the incidence of the influenza virus between 2000 and 2003 in Curitiba. We studied 1621 samples obtained from outpatients and hospitalized patients of both sexes and all ages. The study was conducted at the local primary care health units (outpatients) and at the tertiary care unit (hospitalized) of the General Hospital of the Federal University in the state of Paraná, Brazil. Nasopharyngeal aspirates and, eventually, bronchoalveolar lavage were assayed for the presence of viral antigens, either by indirect immunofluorescence or cell culture. Of the samples studied, 135 (8.3%) were positive for influenza virus, and of those, 103 (76.3%) were positive for type A and 32 (23.7%) for type B. Additionally, positive samples were analyzed by reverse transcription followed by polymerase chain reaction and subtypes H1 and H3 were identified from this group. A high incidence of positive samples was observed mainly in the months with lower temperatures. Furthermore, outpatients showed a higher incidence of influenza viruses than hospitalized patients. |
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The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazilinfluenza virusincidenceseasonalityantigenic characterizationSeveral studies conducted all over the world have reported that the influenza virus is associated with great morbidity and mortality rates. In this study, we analyzed the incidence of the influenza virus between 2000 and 2003 in Curitiba. We studied 1621 samples obtained from outpatients and hospitalized patients of both sexes and all ages. The study was conducted at the local primary care health units (outpatients) and at the tertiary care unit (hospitalized) of the General Hospital of the Federal University in the state of Paraná, Brazil. Nasopharyngeal aspirates and, eventually, bronchoalveolar lavage were assayed for the presence of viral antigens, either by indirect immunofluorescence or cell culture. Of the samples studied, 135 (8.3%) were positive for influenza virus, and of those, 103 (76.3%) were positive for type A and 32 (23.7%) for type B. Additionally, positive samples were analyzed by reverse transcription followed by polymerase chain reaction and subtypes H1 and H3 were identified from this group. A high incidence of positive samples was observed mainly in the months with lower temperatures. Furthermore, outpatients showed a higher incidence of influenza viruses than hospitalized patients.Instituto Oswaldo Cruz, Ministério da Saúde2008-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200009Memórias do Instituto Oswaldo Cruz v.103 n.2 2008reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762008000200009info:eu-repo/semantics/openAccessVidal,Luine RRSiqueira,Marilda MNogueira,Meri BRaboni,Sonia MPereira,Luciane ATakahashi,Gislene RARotta,IndianaraDebur,Maria do CarmoDalla-Costa,Libera Meng2020-04-25T17:50:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:31.238Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
title |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
spellingShingle |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil Vidal,Luine RR influenza virus incidence seasonality antigenic characterization |
title_short |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
title_full |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
title_fullStr |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
title_full_unstemmed |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
title_sort |
The epidemiology and antigenic characterization of influenza viruses isolated in Curitiba, South Brazil |
author |
Vidal,Luine RR |
author_facet |
Vidal,Luine RR Siqueira,Marilda M Nogueira,Meri B Raboni,Sonia M Pereira,Luciane A Takahashi,Gislene RA Rotta,Indianara Debur,Maria do Carmo Dalla-Costa,Libera M |
author_role |
author |
author2 |
Siqueira,Marilda M Nogueira,Meri B Raboni,Sonia M Pereira,Luciane A Takahashi,Gislene RA Rotta,Indianara Debur,Maria do Carmo Dalla-Costa,Libera M |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vidal,Luine RR Siqueira,Marilda M Nogueira,Meri B Raboni,Sonia M Pereira,Luciane A Takahashi,Gislene RA Rotta,Indianara Debur,Maria do Carmo Dalla-Costa,Libera M |
dc.subject.por.fl_str_mv |
influenza virus incidence seasonality antigenic characterization |
topic |
influenza virus incidence seasonality antigenic characterization |
dc.description.none.fl_txt_mv |
Several studies conducted all over the world have reported that the influenza virus is associated with great morbidity and mortality rates. In this study, we analyzed the incidence of the influenza virus between 2000 and 2003 in Curitiba. We studied 1621 samples obtained from outpatients and hospitalized patients of both sexes and all ages. The study was conducted at the local primary care health units (outpatients) and at the tertiary care unit (hospitalized) of the General Hospital of the Federal University in the state of Paraná, Brazil. Nasopharyngeal aspirates and, eventually, bronchoalveolar lavage were assayed for the presence of viral antigens, either by indirect immunofluorescence or cell culture. Of the samples studied, 135 (8.3%) were positive for influenza virus, and of those, 103 (76.3%) were positive for type A and 32 (23.7%) for type B. Additionally, positive samples were analyzed by reverse transcription followed by polymerase chain reaction and subtypes H1 and H3 were identified from this group. A high incidence of positive samples was observed mainly in the months with lower temperatures. Furthermore, outpatients showed a higher incidence of influenza viruses than hospitalized patients. |
description |
Several studies conducted all over the world have reported that the influenza virus is associated with great morbidity and mortality rates. In this study, we analyzed the incidence of the influenza virus between 2000 and 2003 in Curitiba. We studied 1621 samples obtained from outpatients and hospitalized patients of both sexes and all ages. The study was conducted at the local primary care health units (outpatients) and at the tertiary care unit (hospitalized) of the General Hospital of the Federal University in the state of Paraná, Brazil. Nasopharyngeal aspirates and, eventually, bronchoalveolar lavage were assayed for the presence of viral antigens, either by indirect immunofluorescence or cell culture. Of the samples studied, 135 (8.3%) were positive for influenza virus, and of those, 103 (76.3%) were positive for type A and 32 (23.7%) for type B. Additionally, positive samples were analyzed by reverse transcription followed by polymerase chain reaction and subtypes H1 and H3 were identified from this group. A high incidence of positive samples was observed mainly in the months with lower temperatures. Furthermore, outpatients showed a higher incidence of influenza viruses than hospitalized patients. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200009 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762008000200009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.103 n.2 2008 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937701921488896 |