Interaction of avirulent Leishmania species with rat peritoneal macrophages

Detalhes bibliográficos
Autor(a) principal: Bastardo,Trina
Data de Publicação: 1983
Outros Autores: Arcay-de-Peraza,Lucila, Tejero,Félix
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761983000100003
Resumo: An "in vitro" system has been developed for study of host cell-parasite interaction in visceral and cutaneous leishmaniasis. Avirulent promastigotes of L. brasiliensis and L. donovani, from strains originally isolated from human cases and mantained by serial culture in Davis' Medium were allowed to infect cultured macrophages from rat peritoneal exudate. Challenge of the macrophages by parasites took place in 199 medium, at 33ºC for L. brasiliensis and at 37ºC for L. donovani. Although the rat is resistant to infections by Leishmania spp., the promastigotes not only invaded the host cells, but transformed into amastigotes and later mutiplied, from 10 min after challenge to 24 hours later.
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spelling Interaction of avirulent Leishmania species with rat peritoneal macrophagesAn "in vitro" system has been developed for study of host cell-parasite interaction in visceral and cutaneous leishmaniasis. Avirulent promastigotes of L. brasiliensis and L. donovani, from strains originally isolated from human cases and mantained by serial culture in Davis' Medium were allowed to infect cultured macrophages from rat peritoneal exudate. Challenge of the macrophages by parasites took place in 199 medium, at 33ºC for L. brasiliensis and at 37ºC for L. donovani. Although the rat is resistant to infections by Leishmania spp., the promastigotes not only invaded the host cells, but transformed into amastigotes and later mutiplied, from 10 min after challenge to 24 hours later.Instituto Oswaldo Cruz, Ministério da Saúde1983-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761983000100003Memórias do Instituto Oswaldo Cruz v.78 n.1 1983reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761983000100003info:eu-repo/semantics/openAccessBastardo,TrinaArcay-de-Peraza,LucilaTejero,Félixeng2020-04-25T17:45:28Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:00:28.945Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Interaction of avirulent Leishmania species with rat peritoneal macrophages
title Interaction of avirulent Leishmania species with rat peritoneal macrophages
spellingShingle Interaction of avirulent Leishmania species with rat peritoneal macrophages
Bastardo,Trina
title_short Interaction of avirulent Leishmania species with rat peritoneal macrophages
title_full Interaction of avirulent Leishmania species with rat peritoneal macrophages
title_fullStr Interaction of avirulent Leishmania species with rat peritoneal macrophages
title_full_unstemmed Interaction of avirulent Leishmania species with rat peritoneal macrophages
title_sort Interaction of avirulent Leishmania species with rat peritoneal macrophages
author Bastardo,Trina
author_facet Bastardo,Trina
Arcay-de-Peraza,Lucila
Tejero,Félix
author_role author
author2 Arcay-de-Peraza,Lucila
Tejero,Félix
author2_role author
author
dc.contributor.author.fl_str_mv Bastardo,Trina
Arcay-de-Peraza,Lucila
Tejero,Félix
dc.description.none.fl_txt_mv An "in vitro" system has been developed for study of host cell-parasite interaction in visceral and cutaneous leishmaniasis. Avirulent promastigotes of L. brasiliensis and L. donovani, from strains originally isolated from human cases and mantained by serial culture in Davis' Medium were allowed to infect cultured macrophages from rat peritoneal exudate. Challenge of the macrophages by parasites took place in 199 medium, at 33ºC for L. brasiliensis and at 37ºC for L. donovani. Although the rat is resistant to infections by Leishmania spp., the promastigotes not only invaded the host cells, but transformed into amastigotes and later mutiplied, from 10 min after challenge to 24 hours later.
description An "in vitro" system has been developed for study of host cell-parasite interaction in visceral and cutaneous leishmaniasis. Avirulent promastigotes of L. brasiliensis and L. donovani, from strains originally isolated from human cases and mantained by serial culture in Davis' Medium were allowed to infect cultured macrophages from rat peritoneal exudate. Challenge of the macrophages by parasites took place in 199 medium, at 33ºC for L. brasiliensis and at 37ºC for L. donovani. Although the rat is resistant to infections by Leishmania spp., the promastigotes not only invaded the host cells, but transformed into amastigotes and later mutiplied, from 10 min after challenge to 24 hours later.
publishDate 1983
dc.date.none.fl_str_mv 1983-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761983000100003
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761983000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761983000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.78 n.1 1983
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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