Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)

Detalhes bibliográficos
Autor(a) principal: Gomes,Y. M.
Data de Publicação: 1988
Outros Autores: Furtado,A. F., Carvalho,A. B. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000400018
Resumo: The haemolymph of Panstrongylus megistus showed a natural lectin activity for a wide range of vertebrate erythocytes. Agglutination was observed against all vertebrate erythrocytes tested (human ABO, duck, rabbit, mouse, sheep, chicken and cow). Cow erythrocytes showed the lowest titre. Concerning human erythrocytes, the lectin activity was similar in the types A+,B+ and AB+ while the highest activity was observed in the type O+. Determination of minimal inhibitory concentrations was carried out with human erythrocytes type O+. Agglutination was inhibited by several carbohydrates (rhamnose. D-galatose, raffinose, D-lactose and D-fucose). Rhamnose wasreported as the strongest inhibitor (0.78mM). The results suggest the presence of more than one lection in the haemolymph of P. megistus.
id FIOCRUZ-4_da709856499b1f0c4686ffa0a949a7a7
oai_identifier_str oai:scielo:S0074-02761988000400018
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)Panstrongylus megistushaemolymphlectincarbohydrate specificityThe haemolymph of Panstrongylus megistus showed a natural lectin activity for a wide range of vertebrate erythocytes. Agglutination was observed against all vertebrate erythrocytes tested (human ABO, duck, rabbit, mouse, sheep, chicken and cow). Cow erythrocytes showed the lowest titre. Concerning human erythrocytes, the lectin activity was similar in the types A+,B+ and AB+ while the highest activity was observed in the type O+. Determination of minimal inhibitory concentrations was carried out with human erythrocytes type O+. Agglutination was inhibited by several carbohydrates (rhamnose. D-galatose, raffinose, D-lactose and D-fucose). Rhamnose wasreported as the strongest inhibitor (0.78mM). The results suggest the presence of more than one lection in the haemolymph of P. megistus.Instituto Oswaldo Cruz, Ministério da Saúde1988-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000400018Memórias do Instituto Oswaldo Cruz v.83 n.4 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000400018info:eu-repo/semantics/openAccessGomes,Y. M.Furtado,A. F.Carvalho,A. B. deeng2020-04-25T17:46:09Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:33.867Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
title Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
spellingShingle Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
Gomes,Y. M.
Panstrongylus megistus
haemolymph
lectin
carbohydrate specificity
title_short Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
title_full Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
title_fullStr Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
title_full_unstemmed Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
title_sort Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)
author Gomes,Y. M.
author_facet Gomes,Y. M.
Furtado,A. F.
Carvalho,A. B. de
author_role author
author2 Furtado,A. F.
Carvalho,A. B. de
author2_role author
author
dc.contributor.author.fl_str_mv Gomes,Y. M.
Furtado,A. F.
Carvalho,A. B. de
dc.subject.por.fl_str_mv Panstrongylus megistus
haemolymph
lectin
carbohydrate specificity
topic Panstrongylus megistus
haemolymph
lectin
carbohydrate specificity
dc.description.none.fl_txt_mv The haemolymph of Panstrongylus megistus showed a natural lectin activity for a wide range of vertebrate erythocytes. Agglutination was observed against all vertebrate erythrocytes tested (human ABO, duck, rabbit, mouse, sheep, chicken and cow). Cow erythrocytes showed the lowest titre. Concerning human erythrocytes, the lectin activity was similar in the types A+,B+ and AB+ while the highest activity was observed in the type O+. Determination of minimal inhibitory concentrations was carried out with human erythrocytes type O+. Agglutination was inhibited by several carbohydrates (rhamnose. D-galatose, raffinose, D-lactose and D-fucose). Rhamnose wasreported as the strongest inhibitor (0.78mM). The results suggest the presence of more than one lection in the haemolymph of P. megistus.
description The haemolymph of Panstrongylus megistus showed a natural lectin activity for a wide range of vertebrate erythocytes. Agglutination was observed against all vertebrate erythrocytes tested (human ABO, duck, rabbit, mouse, sheep, chicken and cow). Cow erythrocytes showed the lowest titre. Concerning human erythrocytes, the lectin activity was similar in the types A+,B+ and AB+ while the highest activity was observed in the type O+. Determination of minimal inhibitory concentrations was carried out with human erythrocytes type O+. Agglutination was inhibited by several carbohydrates (rhamnose. D-galatose, raffinose, D-lactose and D-fucose). Rhamnose wasreported as the strongest inhibitor (0.78mM). The results suggest the presence of more than one lection in the haemolymph of P. megistus.
publishDate 1988
dc.date.none.fl_str_mv 1988-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000400018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000400018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761988000400018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.83 n.4 1988
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937652208500736