Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400019 |
Resumo: | Passage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction. |
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Memórias do Instituto Oswaldo Cruz |
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Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settingsglass beadsCF l l celluloseleukocytesmalaria-infected human bloodPassage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction.Instituto Oswaldo Cruz, Ministério da Saúde1992-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400019Memórias do Instituto Oswaldo Cruz v.87 n.4 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000400019info:eu-repo/semantics/openAccessGoldman,Ira F.Qari,Shoukat H.Skinner,JimmieOliveira,SalmaNascimento,José M.Póvoa,Marinete M.Collins,William E.Lal,Altaf A.eng2020-04-25T17:46:53Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:48.519Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
title |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
spellingShingle |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings Goldman,Ira F. glass beads CF l l cellulose leukocytes malaria-infected human blood |
title_short |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
title_full |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
title_fullStr |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
title_full_unstemmed |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
title_sort |
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings |
author |
Goldman,Ira F. |
author_facet |
Goldman,Ira F. Qari,Shoukat H. Skinner,Jimmie Oliveira,Salma Nascimento,José M. Póvoa,Marinete M. Collins,William E. Lal,Altaf A. |
author_role |
author |
author2 |
Qari,Shoukat H. Skinner,Jimmie Oliveira,Salma Nascimento,José M. Póvoa,Marinete M. Collins,William E. Lal,Altaf A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Goldman,Ira F. Qari,Shoukat H. Skinner,Jimmie Oliveira,Salma Nascimento,José M. Póvoa,Marinete M. Collins,William E. Lal,Altaf A. |
dc.subject.por.fl_str_mv |
glass beads CF l l cellulose leukocytes malaria-infected human blood |
topic |
glass beads CF l l cellulose leukocytes malaria-infected human blood |
dc.description.none.fl_txt_mv |
Passage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction. |
description |
Passage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400019 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761992000400019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.87 n.4 1992 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937658383564800 |