The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii

Detalhes bibliográficos
Autor(a) principal: Ghafourian,Sobhan
Data de Publicação: 2014
Outros Autores: Good,Liam, Sekawi,Zamberi, Hamat,Rukman Awang, Soheili,Sara, Sadeghifard,Nourkhoda, Neela,Vasanthakumari
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400502
Resumo: Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.
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spelling The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumanniitoxin-antitoxin systemsAcinetobacter baumanniiantibacterial targetAlthough analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.Instituto Oswaldo Cruz, Ministério da Saúde2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400502Memórias do Instituto Oswaldo Cruz v.109 n.4 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276130601info:eu-repo/semantics/openAccessGhafourian,SobhanGood,LiamSekawi,ZamberiHamat,Rukman AwangSoheili,SaraSadeghifard,NourkhodaNeela,Vasanthakumarieng2020-04-25T17:51:51Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:05.237Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
title The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
spellingShingle The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
Ghafourian,Sobhan
toxin-antitoxin systems
Acinetobacter baumannii
antibacterial target
title_short The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
title_full The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
title_fullStr The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
title_full_unstemmed The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
title_sort The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
author Ghafourian,Sobhan
author_facet Ghafourian,Sobhan
Good,Liam
Sekawi,Zamberi
Hamat,Rukman Awang
Soheili,Sara
Sadeghifard,Nourkhoda
Neela,Vasanthakumari
author_role author
author2 Good,Liam
Sekawi,Zamberi
Hamat,Rukman Awang
Soheili,Sara
Sadeghifard,Nourkhoda
Neela,Vasanthakumari
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ghafourian,Sobhan
Good,Liam
Sekawi,Zamberi
Hamat,Rukman Awang
Soheili,Sara
Sadeghifard,Nourkhoda
Neela,Vasanthakumari
dc.subject.por.fl_str_mv toxin-antitoxin systems
Acinetobacter baumannii
antibacterial target
topic toxin-antitoxin systems
Acinetobacter baumannii
antibacterial target
dc.description.none.fl_txt_mv Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.
description Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400502
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400502
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276130601
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.109 n.4 2014
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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