The haemoculture of Trypanosoma minasense chagas, 1908

Detalhes bibliográficos
Autor(a) principal: Ziccardi,Mariangela
Data de Publicação: 1996
Outros Autores: Lourenço-de-Oliveira,Ricardo, Nogueira,Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019
Resumo: Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
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spelling The haemoculture of Trypanosoma minasense chagas, 1908Trypanosoma minasenseneotropical primatestrypanosomesmarmosetstrypanosomatidae flagellatescultureBrazilTrypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.Instituto Oswaldo Cruz, Ministério da Saúde1996-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019Memórias do Instituto Oswaldo Cruz v.91 n.4 1996reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761996000400019info:eu-repo/semantics/openAccessZiccardi,MariangelaLourenço-de-Oliveira,RicardoNogueira,Ricardoeng2020-04-25T17:47:35Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:06.767Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The haemoculture of Trypanosoma minasense chagas, 1908
title The haemoculture of Trypanosoma minasense chagas, 1908
spellingShingle The haemoculture of Trypanosoma minasense chagas, 1908
Ziccardi,Mariangela
Trypanosoma minasense
neotropical primates
trypanosomes
marmosets
trypanosomatidae flagellates
culture
Brazil
title_short The haemoculture of Trypanosoma minasense chagas, 1908
title_full The haemoculture of Trypanosoma minasense chagas, 1908
title_fullStr The haemoculture of Trypanosoma minasense chagas, 1908
title_full_unstemmed The haemoculture of Trypanosoma minasense chagas, 1908
title_sort The haemoculture of Trypanosoma minasense chagas, 1908
author Ziccardi,Mariangela
author_facet Ziccardi,Mariangela
Lourenço-de-Oliveira,Ricardo
Nogueira,Ricardo
author_role author
author2 Lourenço-de-Oliveira,Ricardo
Nogueira,Ricardo
author2_role author
author
dc.contributor.author.fl_str_mv Ziccardi,Mariangela
Lourenço-de-Oliveira,Ricardo
Nogueira,Ricardo
dc.subject.por.fl_str_mv Trypanosoma minasense
neotropical primates
trypanosomes
marmosets
trypanosomatidae flagellates
culture
Brazil
topic Trypanosoma minasense
neotropical primates
trypanosomes
marmosets
trypanosomatidae flagellates
culture
Brazil
dc.description.none.fl_txt_mv Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
description Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
publishDate 1996
dc.date.none.fl_str_mv 1996-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761996000400019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.91 n.4 1996
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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