IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis

Detalhes bibliográficos
Autor(a) principal: Machado,Anderson S
Data de Publicação: 2010
Outros Autores: Andrade,Gláucia MQ, Januário,José N, Fernandes,Matheus D, Carneiro,Ana Carolina AV, Carneiro,Mariângela, Carellos,Ericka VM, Romanelli,Roberta MC, Vasconcelos-Santos,Daniel V, Vitor,Ricardo WA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005
Resumo: The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.
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spelling IgG and IgM western blot assay for diagnosis of congenital toxoplasmosiscongenital toxoplasmosiswestern blotmacular toxoplasmosisThe aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.Instituto Oswaldo Cruz, Ministério da Saúde2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005Memórias do Instituto Oswaldo Cruz v.105 n.6 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000600005info:eu-repo/semantics/openAccessMachado,Anderson SAndrade,Gláucia MQJanuário,José NFernandes,Matheus DCarneiro,Ana Carolina AVCarneiro,MariângelaCarellos,Ericka VMRomanelli,Roberta MCVasconcelos-Santos,Daniel VVitor,Ricardo WAeng2020-04-25T17:50:50Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:13.982Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
title IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
spellingShingle IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
Machado,Anderson S
congenital toxoplasmosis
western blot
macular toxoplasmosis
title_short IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
title_full IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
title_fullStr IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
title_full_unstemmed IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
title_sort IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
author Machado,Anderson S
author_facet Machado,Anderson S
Andrade,Gláucia MQ
Januário,José N
Fernandes,Matheus D
Carneiro,Ana Carolina AV
Carneiro,Mariângela
Carellos,Ericka VM
Romanelli,Roberta MC
Vasconcelos-Santos,Daniel V
Vitor,Ricardo WA
author_role author
author2 Andrade,Gláucia MQ
Januário,José N
Fernandes,Matheus D
Carneiro,Ana Carolina AV
Carneiro,Mariângela
Carellos,Ericka VM
Romanelli,Roberta MC
Vasconcelos-Santos,Daniel V
Vitor,Ricardo WA
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Machado,Anderson S
Andrade,Gláucia MQ
Januário,José N
Fernandes,Matheus D
Carneiro,Ana Carolina AV
Carneiro,Mariângela
Carellos,Ericka VM
Romanelli,Roberta MC
Vasconcelos-Santos,Daniel V
Vitor,Ricardo WA
dc.subject.por.fl_str_mv congenital toxoplasmosis
western blot
macular toxoplasmosis
topic congenital toxoplasmosis
western blot
macular toxoplasmosis
dc.description.none.fl_txt_mv The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.
description The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.6 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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