Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera

Detalhes bibliográficos
Autor(a) principal: Morgado,M. G.
Data de Publicação: 1989
Outros Autores: Ivo-dos-Santos,J., Pinho,R. T., Argüelles,E., Rezende,J. M., Galvão-Castro,B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300004
Resumo: Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.
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spelling Trypanosoma cruzi: identification of specific epimastigote antigens by human immune serawestern blotTrypanosoma cruziantigensspecifitycross-reactivitySoluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.Instituto Oswaldo Cruz, Ministério da Saúde1989-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300004Memórias do Instituto Oswaldo Cruz v.84 n.3 1989reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761989000300004info:eu-repo/semantics/openAccessMorgado,M. G.Ivo-dos-Santos,J.Pinho,R. T.Argüelles,E.Rezende,J. M.Galvão-Castro,B.eng2020-04-25T17:46:16Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:58.823Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
title Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
spellingShingle Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
Morgado,M. G.
western blot
Trypanosoma cruzi
antigens
specifity
cross-reactivity
title_short Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
title_full Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
title_fullStr Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
title_full_unstemmed Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
title_sort Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
author Morgado,M. G.
author_facet Morgado,M. G.
Ivo-dos-Santos,J.
Pinho,R. T.
Argüelles,E.
Rezende,J. M.
Galvão-Castro,B.
author_role author
author2 Ivo-dos-Santos,J.
Pinho,R. T.
Argüelles,E.
Rezende,J. M.
Galvão-Castro,B.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Morgado,M. G.
Ivo-dos-Santos,J.
Pinho,R. T.
Argüelles,E.
Rezende,J. M.
Galvão-Castro,B.
dc.subject.por.fl_str_mv western blot
Trypanosoma cruzi
antigens
specifity
cross-reactivity
topic western blot
Trypanosoma cruzi
antigens
specifity
cross-reactivity
dc.description.none.fl_txt_mv Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.
description Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.
publishDate 1989
dc.date.none.fl_str_mv 1989-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300004
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761989000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.84 n.3 1989
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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