The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500008 |
Resumo: | The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species. |
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Memórias do Instituto Oswaldo Cruz |
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The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence GenesVibrio choleraeAmazoniatoxRouter membrane proteinsproteaseLonThe pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species.Instituto Oswaldo Cruz, Ministério da Saúde1998-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500008Memórias do Instituto Oswaldo Cruz v.93 n.5 1998reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761998000500008info:eu-repo/semantics/openAccessBaptista,MASAndrade,JRCVicente,ACPSalles,CACoelho,Aeng2020-04-25T17:47:54Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:08:15.704Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
title |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
spellingShingle |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes Baptista,MAS Vibrio cholerae Amazonia toxR outer membrane proteins protease Lon |
title_short |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
title_full |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
title_fullStr |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
title_full_unstemmed |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
title_sort |
The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes |
author |
Baptista,MAS |
author_facet |
Baptista,MAS Andrade,JRC Vicente,ACP Salles,CA Coelho,A |
author_role |
author |
author2 |
Andrade,JRC Vicente,ACP Salles,CA Coelho,A |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Baptista,MAS Andrade,JRC Vicente,ACP Salles,CA Coelho,A |
dc.subject.por.fl_str_mv |
Vibrio cholerae Amazonia toxR outer membrane proteins protease Lon |
topic |
Vibrio cholerae Amazonia toxR outer membrane proteins protease Lon |
dc.description.none.fl_txt_mv |
The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species. |
description |
The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500008 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761998000500008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.93 n.5 1998 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937673055240192 |