A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage

Detalhes bibliográficos
Autor(a) principal: Jablonka,Willy
Data de Publicação: 2011
Outros Autores: Senna,Raquel, Nahu,Thaisa, Ventura,Guilherme, Menezes,Lidiane, Silva-Neto,Mário Alberto C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500005
Resumo: Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.
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spelling A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stageAedes aegyptityrosine phosphorylationblood-feedingPhosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.Instituto Oswaldo Cruz, Ministério da Saúde2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500005Memórias do Instituto Oswaldo Cruz v.106 n.5 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000500005info:eu-repo/semantics/openAccessJablonka,WillySenna,RaquelNahu,ThaisaVentura,GuilhermeMenezes,LidianeSilva-Neto,Mário Alberto Ceng2020-04-25T17:51:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:44.362Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
title A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
spellingShingle A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
Jablonka,Willy
Aedes aegypti
tyrosine phosphorylation
blood-feeding
title_short A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
title_full A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
title_fullStr A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
title_full_unstemmed A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
title_sort A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage
author Jablonka,Willy
author_facet Jablonka,Willy
Senna,Raquel
Nahu,Thaisa
Ventura,Guilherme
Menezes,Lidiane
Silva-Neto,Mário Alberto C
author_role author
author2 Senna,Raquel
Nahu,Thaisa
Ventura,Guilherme
Menezes,Lidiane
Silva-Neto,Mário Alberto C
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Jablonka,Willy
Senna,Raquel
Nahu,Thaisa
Ventura,Guilherme
Menezes,Lidiane
Silva-Neto,Mário Alberto C
dc.subject.por.fl_str_mv Aedes aegypti
tyrosine phosphorylation
blood-feeding
topic Aedes aegypti
tyrosine phosphorylation
blood-feeding
dc.description.none.fl_txt_mv Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.
description Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000500005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.5 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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