Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library

Detalhes bibliográficos
Autor(a) principal: Franco,Glória R.
Data de Publicação: 1995
Outros Autores: Simpson,Andrew J. G., Pena,Sérgio D. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200015
Resumo: We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
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spelling Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional librarySchistosoma mansoniautomated DNA sequencingESTgene identificationWe have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.Instituto Oswaldo Cruz, Ministério da Saúde1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200015Memórias do Instituto Oswaldo Cruz v.90 n.2 1995reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761995000200015info:eu-repo/semantics/openAccessFranco,Glória R.Simpson,Andrew J. G.Pena,Sérgio D. J.eng2020-04-25T17:47:23Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:27.339Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
title Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
spellingShingle Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
Franco,Glória R.
Schistosoma mansoni
automated DNA sequencing
EST
gene identification
title_short Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
title_full Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
title_fullStr Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
title_full_unstemmed Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
title_sort Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
author Franco,Glória R.
author_facet Franco,Glória R.
Simpson,Andrew J. G.
Pena,Sérgio D. J.
author_role author
author2 Simpson,Andrew J. G.
Pena,Sérgio D. J.
author2_role author
author
dc.contributor.author.fl_str_mv Franco,Glória R.
Simpson,Andrew J. G.
Pena,Sérgio D. J.
dc.subject.por.fl_str_mv Schistosoma mansoni
automated DNA sequencing
EST
gene identification
topic Schistosoma mansoni
automated DNA sequencing
EST
gene identification
dc.description.none.fl_txt_mv We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
description We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
publishDate 1995
dc.date.none.fl_str_mv 1995-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200015
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761995000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.90 n.2 1995
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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