Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

Detalhes bibliográficos
Autor(a) principal: Gentil,Luciana Girotto
Data de Publicação: 2007
Outros Autores: Lasakosvitsch,Fernanda, Silveira,José Franco da, Santos,Márcia Regina Machado dos, Barbiéri,Clara Lúcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600008
Resumo: The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.
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spelling Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tagsLeishmania (Leishmania) amazonensisamastigotescDNA libraryThe characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.Instituto Oswaldo Cruz, Ministério da Saúde2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600008Memórias do Instituto Oswaldo Cruz v.102 n.6 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000065info:eu-repo/semantics/openAccessGentil,Luciana GirottoLasakosvitsch,FernandaSilveira,José Franco daSantos,Márcia Regina Machado dosBarbiéri,Clara Lúciaeng2020-04-25T17:50:09Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:13.991Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
title Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
spellingShingle Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
Gentil,Luciana Girotto
Leishmania (Leishmania) amazonensis
amastigotes
cDNA library
title_short Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
title_full Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
title_fullStr Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
title_full_unstemmed Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
title_sort Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
author Gentil,Luciana Girotto
author_facet Gentil,Luciana Girotto
Lasakosvitsch,Fernanda
Silveira,José Franco da
Santos,Márcia Regina Machado dos
Barbiéri,Clara Lúcia
author_role author
author2 Lasakosvitsch,Fernanda
Silveira,José Franco da
Santos,Márcia Regina Machado dos
Barbiéri,Clara Lúcia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gentil,Luciana Girotto
Lasakosvitsch,Fernanda
Silveira,José Franco da
Santos,Márcia Regina Machado dos
Barbiéri,Clara Lúcia
dc.subject.por.fl_str_mv Leishmania (Leishmania) amazonensis
amastigotes
cDNA library
topic Leishmania (Leishmania) amazonensis
amastigotes
cDNA library
dc.description.none.fl_txt_mv The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.
description The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600008
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000600008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762007005000065
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.102 n.6 2007
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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