A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

Detalhes bibliográficos
Autor(a) principal: Coban,Ahmet Yilmaz
Data de Publicação: 2011
Outros Autores: Cayci,Yeliz Tanriverdi, Deveci,Aydin, Akgunes,Alper, Uzun,Meltem, Durupinar,Belma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300022
Resumo: The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.
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spelling A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agarMycobacterium tuberculosisnitrate reductase assayblood agarThe susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.Instituto Oswaldo Cruz, Ministério da Saúde2011-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300022Memórias do Instituto Oswaldo Cruz v.106 n.3 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000300022info:eu-repo/semantics/openAccessCoban,Ahmet YilmazCayci,Yeliz TanriverdiDeveci,AydinAkgunes,AlperUzun,MeltemDurupinar,Belmaeng2020-04-25T17:50:59Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:38.765Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
title A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
spellingShingle A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
Coban,Ahmet Yilmaz
Mycobacterium tuberculosis
nitrate reductase assay
blood agar
title_short A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
title_full A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
title_fullStr A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
title_full_unstemmed A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
title_sort A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar
author Coban,Ahmet Yilmaz
author_facet Coban,Ahmet Yilmaz
Cayci,Yeliz Tanriverdi
Deveci,Aydin
Akgunes,Alper
Uzun,Meltem
Durupinar,Belma
author_role author
author2 Cayci,Yeliz Tanriverdi
Deveci,Aydin
Akgunes,Alper
Uzun,Meltem
Durupinar,Belma
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Coban,Ahmet Yilmaz
Cayci,Yeliz Tanriverdi
Deveci,Aydin
Akgunes,Alper
Uzun,Meltem
Durupinar,Belma
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
nitrate reductase assay
blood agar
topic Mycobacterium tuberculosis
nitrate reductase assay
blood agar
dc.description.none.fl_txt_mv The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.
description The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.
publishDate 2011
dc.date.none.fl_str_mv 2011-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300022
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000300022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.3 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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