Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement

Detalhes bibliográficos
Autor(a) principal: Vital-Brazil,Juliana Magalhães
Data de Publicação: 2010
Outros Autores: Balassiano,Ilana Teruszkin, Oliveira,Fabiano Sutter de, Costa,Alberto Dias de Souza, Hillen,Leandro, Pereira,Martha Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020
Resumo: The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
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spelling Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlementLeptospira sppmultiplex PCRwater sampleThe aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.Instituto Oswaldo Cruz, Ministério da Saúde2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020Memórias do Instituto Oswaldo Cruz v.105 n.3 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000300020info:eu-repo/semantics/openAccessVital-Brazil,Juliana MagalhãesBalassiano,Ilana TeruszkinOliveira,Fabiano Sutter deCosta,Alberto Dias de SouzaHillen,LeandroPereira,Martha Mariaeng2020-04-25T17:50:47Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:56.726Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
title Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
spellingShingle Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
Vital-Brazil,Juliana Magalhães
Leptospira spp
multiplex PCR
water sample
title_short Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
title_full Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
title_fullStr Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
title_full_unstemmed Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
title_sort Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
author Vital-Brazil,Juliana Magalhães
author_facet Vital-Brazil,Juliana Magalhães
Balassiano,Ilana Teruszkin
Oliveira,Fabiano Sutter de
Costa,Alberto Dias de Souza
Hillen,Leandro
Pereira,Martha Maria
author_role author
author2 Balassiano,Ilana Teruszkin
Oliveira,Fabiano Sutter de
Costa,Alberto Dias de Souza
Hillen,Leandro
Pereira,Martha Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vital-Brazil,Juliana Magalhães
Balassiano,Ilana Teruszkin
Oliveira,Fabiano Sutter de
Costa,Alberto Dias de Souza
Hillen,Leandro
Pereira,Martha Maria
dc.subject.por.fl_str_mv Leptospira spp
multiplex PCR
water sample
topic Leptospira spp
multiplex PCR
water sample
dc.description.none.fl_txt_mv The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
description The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000300020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.3 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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