Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures

Detalhes bibliográficos
Autor(a) principal: Barth,Ortrud Monika
Data de Publicação: 1988
Outros Autores: Majerowicz,Selma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100009
Resumo: Transmission electon microscopy has been employed for the rapid detection of mycoplasma in sera and cell cultures. High speed centrifugation of sera or low speed centrifugation of cell debris, followed by negative staining of the resuspended pellet, detected mycoplasma contamination more frequently than a culture method followed by direct fluorescence (DAPI), which was used as a control procedure. The appearance of the mycoplasma cell border and content gives some information about particle viability.
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spelling Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell culturesmycoplasmasnegative contrastrapid methodsera and cell contaminationTransmission electon microscopy has been employed for the rapid detection of mycoplasma in sera and cell cultures. High speed centrifugation of sera or low speed centrifugation of cell debris, followed by negative staining of the resuspended pellet, detected mycoplasma contamination more frequently than a culture method followed by direct fluorescence (DAPI), which was used as a control procedure. The appearance of the mycoplasma cell border and content gives some information about particle viability.Instituto Oswaldo Cruz, Ministério da Saúde1988-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100009Memórias do Instituto Oswaldo Cruz v.83 n.1 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000100009info:eu-repo/semantics/openAccessBarth,Ortrud MonikaMajerowicz,Selmaeng2020-04-25T17:46:04Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:19.826Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
title Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
spellingShingle Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
Barth,Ortrud Monika
mycoplasmas
negative contrast
rapid method
sera and cell contamination
title_short Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
title_full Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
title_fullStr Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
title_full_unstemmed Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
title_sort Rapid detection by transmission electron microscopy of mycoplasma contamination in sera and cell cultures
author Barth,Ortrud Monika
author_facet Barth,Ortrud Monika
Majerowicz,Selma
author_role author
author2 Majerowicz,Selma
author2_role author
dc.contributor.author.fl_str_mv Barth,Ortrud Monika
Majerowicz,Selma
dc.subject.por.fl_str_mv mycoplasmas
negative contrast
rapid method
sera and cell contamination
topic mycoplasmas
negative contrast
rapid method
sera and cell contamination
dc.description.none.fl_txt_mv Transmission electon microscopy has been employed for the rapid detection of mycoplasma in sera and cell cultures. High speed centrifugation of sera or low speed centrifugation of cell debris, followed by negative staining of the resuspended pellet, detected mycoplasma contamination more frequently than a culture method followed by direct fluorescence (DAPI), which was used as a control procedure. The appearance of the mycoplasma cell border and content gives some information about particle viability.
description Transmission electon microscopy has been employed for the rapid detection of mycoplasma in sera and cell cultures. High speed centrifugation of sera or low speed centrifugation of cell debris, followed by negative staining of the resuspended pellet, detected mycoplasma contamination more frequently than a culture method followed by direct fluorescence (DAPI), which was used as a control procedure. The appearance of the mycoplasma cell border and content gives some information about particle viability.
publishDate 1988
dc.date.none.fl_str_mv 1988-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761988000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.83 n.1 1988
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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