Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region

Detalhes bibliográficos
Autor(a) principal: Viana, Giselle Maria Rachid
Data de Publicação: 2006
Outros Autores: Machado, Ricardo Luís Dantas, Calvosa, Vanja Sueli Pachiano, Póvoa, Marinete Marins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Cadernos de Saúde Pública
Texto Completo: https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831
Resumo: The objectives of this study were to investigate the molecular basis for Plasmodium falciparum resistance to chloroquine in isolates from the Brazilian Amazon and to identify polymorphisms in the pfmdr1 gene, codons 184, 1042, and 1246, the kappa and gamma regions of the cg2 gene, and the K76T mutation of the pfcrt gene, in order to calculate the distribution of polymorphism within each target gene, comparing samples from distinct geographic areas, using allele-specific polymerase chain reaction (PCR) for the pfmdr gene and PCR plus restriction fragment length polymorphism (RFLP) for the cg2 and pfcrt genes. The sample consisted of 40 human blood isolates, already collected and morphologically diagnosed as carriers of P. falciparum parasites, from four localities: Porto Velho in Rondonia State and Maraba, Itaituba, and Tailandia in Pará State. Distribution of P. falciparum in vitro chloroquine resistance in the isolates was 100% for pfmdr1, cg2 gamma region, and pfcrt, except for the polymorphism in the cg2 kappa region, which was not found.
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spelling Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon RegionPlasmodium falciparumGenetic PolymorphismChloroquineThe objectives of this study were to investigate the molecular basis for Plasmodium falciparum resistance to chloroquine in isolates from the Brazilian Amazon and to identify polymorphisms in the pfmdr1 gene, codons 184, 1042, and 1246, the kappa and gamma regions of the cg2 gene, and the K76T mutation of the pfcrt gene, in order to calculate the distribution of polymorphism within each target gene, comparing samples from distinct geographic areas, using allele-specific polymerase chain reaction (PCR) for the pfmdr gene and PCR plus restriction fragment length polymorphism (RFLP) for the cg2 and pfcrt genes. The sample consisted of 40 human blood isolates, already collected and morphologically diagnosed as carriers of P. falciparum parasites, from four localities: Porto Velho in Rondonia State and Maraba, Itaituba, and Tailandia in Pará State. Distribution of P. falciparum in vitro chloroquine resistance in the isolates was 100% for pfmdr1, cg2 gamma region, and pfcrt, except for the polymorphism in the cg2 kappa region, which was not found.O estudo foi desenvolvido para investigar a base molecular da resistência do Plasmodium falciparum à cloroquina em isolados da região Amazônica brasileira e identificar os polimorfismos nos códons TYR184PHE, ASN1042ASP e ASP1246TYR do gene pfmdr1, as regiões kappa e gamma do gene cg2 e a mutação K76T do gene pfcrt, a fim de determinar a distribuição percentual dos alelos de cada gene estudado, comparando amostras de áreas geográficas distintas, utilizando a reação em cadeia da polimerase (PCR) alelo-específica para o pfmdr1 e a PCR e o polimorfismo do comprimento do fragmento de restrição (RFLP) para os genes cg2 e pfcrt. A amostra foi constituída de quarenta isolados de sangue humano já coletados e microscopicamente diagnosticados com malária por P. falciparum das localidades de Porto Velho (Rondônia) e Marabá, Itaituba e Tailândia (Pará). A distribuição percentual da resistência in vitro do P. falciparum à cloroquina nas amostras estudadas foi de 100% de resistência para os genes pfmdr1, região gamma do cg2 e pfcrt. O polimorfismo na região kappa do gene cg2 não foi encontrado nas amostras estudadas.Reports in Public HealthCadernos de Saúde Pública2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlapplication/pdfhttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831Reports in Public Health; Vol. 22 No. 12 (2006): DecemberCadernos de Saúde Pública; v. 22 n. 12 (2006): Dezembro1678-44640102-311Xreponame:Cadernos de Saúde Públicainstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831/5700https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831/5701Viana, Giselle Maria RachidMachado, Ricardo Luís DantasCalvosa, Vanja Sueli PachianoPóvoa, Marinete Marinsinfo:eu-repo/semantics/openAccess2024-03-06T15:27:16Zoai:ojs.teste-cadernos.ensp.fiocruz.br:article/2831Revistahttps://cadernos.ensp.fiocruz.br/ojs/index.php/csphttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/oaicadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br1678-44640102-311Xopendoar:2024-03-06T13:03:17.227692Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)true
dc.title.none.fl_str_mv Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
title Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
spellingShingle Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
Viana, Giselle Maria Rachid
Plasmodium falciparum
Genetic Polymorphism
Chloroquine
title_short Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
title_full Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
title_fullStr Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
title_full_unstemmed Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
title_sort Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
author Viana, Giselle Maria Rachid
author_facet Viana, Giselle Maria Rachid
Machado, Ricardo Luís Dantas
Calvosa, Vanja Sueli Pachiano
Póvoa, Marinete Marins
author_role author
author2 Machado, Ricardo Luís Dantas
Calvosa, Vanja Sueli Pachiano
Póvoa, Marinete Marins
author2_role author
author
author
dc.contributor.author.fl_str_mv Viana, Giselle Maria Rachid
Machado, Ricardo Luís Dantas
Calvosa, Vanja Sueli Pachiano
Póvoa, Marinete Marins
dc.subject.por.fl_str_mv Plasmodium falciparum
Genetic Polymorphism
Chloroquine
topic Plasmodium falciparum
Genetic Polymorphism
Chloroquine
description The objectives of this study were to investigate the molecular basis for Plasmodium falciparum resistance to chloroquine in isolates from the Brazilian Amazon and to identify polymorphisms in the pfmdr1 gene, codons 184, 1042, and 1246, the kappa and gamma regions of the cg2 gene, and the K76T mutation of the pfcrt gene, in order to calculate the distribution of polymorphism within each target gene, comparing samples from distinct geographic areas, using allele-specific polymerase chain reaction (PCR) for the pfmdr gene and PCR plus restriction fragment length polymorphism (RFLP) for the cg2 and pfcrt genes. The sample consisted of 40 human blood isolates, already collected and morphologically diagnosed as carriers of P. falciparum parasites, from four localities: Porto Velho in Rondonia State and Maraba, Itaituba, and Tailandia in Pará State. Distribution of P. falciparum in vitro chloroquine resistance in the isolates was 100% for pfmdr1, cg2 gamma region, and pfcrt, except for the polymorphism in the cg2 kappa region, which was not found.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831
url https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831/5700
https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/2831/5701
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
application/pdf
dc.publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
dc.source.none.fl_str_mv Reports in Public Health; Vol. 22 No. 12 (2006): December
Cadernos de Saúde Pública; v. 22 n. 12 (2006): Dezembro
1678-4464
0102-311X
reponame:Cadernos de Saúde Pública
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Cadernos de Saúde Pública
collection Cadernos de Saúde Pública
repository.name.fl_str_mv Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv cadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br
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